||Recombinant human PSMA8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||PSMA8 belongs to the peptidase T1A family. PSMA8 is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit may be a testis-specific.
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol, 0.1 M NaCl.
||30 kDa (270 aa) confirmed by MALDI-TOF
||> 90 % by SDS-PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MASRYDRAIT VFSPDGHLFQ VEYAQEAVKK GSTAVGIRGT NIVVLGVEKK SVAKLQDERT VRKICALDDH VCMAFAGLTA DARVVINRAR VECQSHKLTV EDPVTVEYIT RFIATLKQKY TQSNGRRPFG ISALIVGFDD DGISRLYQTD PSGTYHAWKA NAIGRSAKTV REFLEKNYTE DAIASDSEAI KLAIKALLEV VQSGGKNIEL AIIRRNQPLK MFSAKEVELY VTEIEKEKEE AEKKKSKKSV
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||APC/C-mediated degradation of cell cycle proteins; APC/C:Cdc20 mediated degradation of Securin; Apoptosis; CDK-mediated phosphorylation and removal of Cdc6; Cell Cycle Checkpoints; DNA Replication; DNA Replication Pre-Initiation; Diurnally regulated genes with circadian orthologs; G1/S DNA Damage Checkpoints; Regulation of APC/C activators between G1/S and early anaphase; Regulation of mitotic cell cycle; SCF-beta-TrCP mediated degradation of Emi1