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Recombinant Human TRNA Phosphotransferase 1, His-tagged

Cat.No. : TRPT1-1327H
Product Overview : Recombinant human TRPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
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Cat. No. : TRPT1-1327H
Description : TRPT1, also known as tRNA 2"-phosphotransferase 1, catalyzes the last step of tRNA splicing, the transfer of the splice junction 2"-phosphate from ligated tRNA to NAD to produce ADP-ribose 1"-2" cyclic phosphate.
Form : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 20% glycerol, 0.1 M NaCl.
Molecular Weight : 30.3 kDa (277 aa), confirmed by MALDI-TOF
Purity : > 95 % by SDS-PAGE
Concentration : 0.25 mg/ml (determined by Bradford assay)
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MGSHMNFSGG GRQEAAGSRG RRAPRPREQD RDVQLSKALS YALRHGALKL GLPMGADGFV PLGTLLQLPQ FRGFSAEDVQ RVVDTNRKQR FALQLGDPST GLLIRANQGH SLQVPKLELM PLETPQALPP MLVHGTFWKH WPSILLKGLS CQGRTHIHLA PGLPGDPGII SGMRSHCEIA VFIDGPLALA DGIPFFRSAN GVILTPGNTD GFLLPKYFKE ALQLRPTRKP LSLAGDEETE CQSSPKHSSR ERRRIQQ
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : TRPT1 tRNA phosphotransferase 1 [ Homo sapiens ]
Official Symbol : TRPT1
Synonyms : TRPT1; tRNA phosphotransferase 1; MGC11134; tRNA 2"-phosphotransferase 1; OTTHUMP00000237038; OTTHUMP00000237040; OTTHUMP00000237336; OTTHUMP00000237493; tRNA splicing 2" phosphotransferase 1; NP_001028850.2; EC 2.7.1.160
Gene ID : 83707
mRNA Refseq : NM_001033678
Protein Refseq : NP_001028850
MIM : 610470
UniProt ID : Q86TN4
Chromosome Location : 11q13
Function : tRNA 2"-phosphotransferase activity; transferase activity, transferring phosphorus-containing groups

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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