"TRPT1" Related Products

Recombinant Human TRNA Phosphotransferase 1, His-tagged

Cat. No.: TRPT1-1327H
Product Overview: Recombinant human TRPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Description: TRPT1, also known as tRNA 2"-phosphotransferase 1, catalyzes the last step of tRNA splicing, the transfer of the splice junction 2"-phosphate from ligated tRNA to NAD to produce ADP-ribose 1"-2" cyclic phosphate.
Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 20% glycerol, 0.1 M NaCl.
Molecular Weight: 30.3 kDa (277 aa), confirmed by MALDI-TOF
Purity: > 95 % by SDS-PAGE
Concentration: 0.25 mg/ml (determined by Bradford assay)
Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMNFSGG GRQEAAGSRG RRAPRPREQD RDVQLSKALS YALRHGALKL GLPMGADGFV PLGTLLQLPQ FRGFSAEDVQ RVVDTNRKQR FALQLGDPST GLLIRANQGH SLQVPKLELM PLETPQALPP MLVHGTFWKH WPSILLKGLS CQGRTHIHLA PGLPGDPGII SGMRSHCEIA VFIDGPLALA DGIPFFRSAN GVILTPGNTD GFLLPKYFKE ALQLRPTRKP LSLAGDEETE CQSSPKHSSR ERRRIQQ
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: TRPT1 tRNA phosphotransferase 1 [ Homo sapiens ]
Official Symbol: TRPT1
Synonyms: TRPT1; tRNA phosphotransferase 1; MGC11134; tRNA 2"-phosphotransferase 1; OTTHUMP00000237038; OTTHUMP00000237040; OTTHUMP00000237336; OTTHUMP00000237493; tRNA splicing 2" phosphotransferase 1; NP_001028850.2; EC 2.7.1.160
Gene ID: 83707
mRNA Refseq: NM_001033678
Protein Refseq: NP_001028850
MIM: 610470
UniProt ID: Q86TN4
Chromosome Location: 11q13
Function: tRNA 2"-phosphotransferase activity; transferase activity, transferring phosphorus-containing groups

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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