||Recombinant human TRPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||TRPT1, also known as tRNA 2"-phosphotransferase 1, catalyzes the last step of tRNA splicing, the transfer of the splice junction 2"-phosphate from ligated tRNA to NAD to produce ADP-ribose 1"-2" cyclic phosphate.
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 20% glycerol, 0.1 M NaCl.
||30.3 kDa (277 aa), confirmed by MALDI-TOF
||> 95 % by SDS-PAGE
||0.25 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MGSHMNFSGG GRQEAAGSRG RRAPRPREQD RDVQLSKALS YALRHGALKL GLPMGADGFV PLGTLLQLPQ FRGFSAEDVQ RVVDTNRKQR FALQLGDPST GLLIRANQGH SLQVPKLELM PLETPQALPP MLVHGTFWKH WPSILLKGLS CQGRTHIHLA PGLPGDPGII SGMRSHCEIA VFIDGPLALA DGIPFFRSAN GVILTPGNTD GFLLPKYFKE ALQLRPTRKP LSLAGDEETE CQSSPKHSSR ERRRIQQ
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.