||Recombinant human PSAT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||PSAT1, also known as phosphoserine aminotransferase, catalyses the conversion of 3-phosphohydroxypyruvate into 3-phosphoserine which is dephosphorylated subsequently by phosphoserine phosphatase to form L-serine.
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 20% glycerol.
||42.9 kDa (394 aa), confirmed by MALDI-TOF
||> 90 % by SDS-PAGE
||1 mg/ml (determined by Bradford)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MGSHMDAPRQ VVNFGPGPAK LPHSVLLEIQ KELLDYKGVG ISVLEMSHRS SDFAKIINNT ENLVRELLAV PDNYKVIFLQ GGGCGQFSAV PLNLIGLKAG RCADYVVTGA WSAKAAEEAK KFGTINIVHP KLGSYTKIPD PSTWNLNPDA SYVYYCANET VHGVEFDFIP DVKGAVLVCD MSSNFLSKPV DVSKFGVIFA GAQKNVGSAG VTVVIVRDDL LGFALRECPS VLEYKVQAGN SSLYNTPPCF SIYVMGLVLE WIKNNGGAAA MEKLSSIKSQ TIYEIIDNSQ GFYVCPVEPQ NRSKMNIPFR IGNAKGDDAL EKRFLDKALE LNMLSLKGHR SVGGIRASLY NAVTIEDVQK LAAFMKKFLE MHQL
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Glycine, serine and threonine metabolism; Metabolic pathways; Vitamin B6 metabolism; Serine biosynthesis