||Recombinant human MRAS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||MRAS (Ras-related protein M-Ras) belongs to the RAS superfamily. These families are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors. MRAS serve as an important signal transducer for novel upstream stimuli in controlling cell proliferation.
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 5 mM DTT, 40% glycerol, 200 mM NaCl, 2 mM EDTA.
||25.7 kDa (225 aa), confirmed by MALDI-TOF
||> 90 % by SDS-PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MATSAVPSDN LPTYKLVVVG DGGVGKSALT IQFFQKIFVP DYDPTIEDSY LKHTEIDNQW AILDVLDTAG QEEFSAMREQ YMRTGDGFLI VYSVTDKASF EHVDRFHQLI LRVKDRESFP MILVANKVDL MHLRKITREQ GKEMATKHNI PYIETSAKDP PLNVDKAFHD LVRVIRQQIP EKSQKKKKKT KWRGDRATGT HKLQC
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||HTLV-I infection; IL-3 Signaling Pathway; MAPK signaling pathway; Regulation of Actin Cytoskeleton; Tight junction