||Recombinant human DHRS9 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||DHRS9, also known as dehydrogenase/reductase SDR family member 9, functions as a homotetramer that converts both 3-alpha-tetrahydroprogesterone (allopregnanolone) and 3-alpha-androstanediol to dihydroxyprogesterone and is thought to play a role in retinoic acid biosynthesis.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 2mM DTT, 0.1mM PMSF.
||35.9 kDa (327aa), confirmed by MALDI-TOF.
||>90% by SDS-PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MGSHMRKGKL KIEDITDKYI FITGCDSGFG NLAARTFDKK GFHVIAACLT ESGSTALKAE TSERLRTVLLDVTDPENVKR TAQWVKNQVG EKGLWGLINN AGVPGVLAPT DWLTLEDYRE PIEVNLFGLI SVTLNMLPLV KKAQGRVINV SSVGGRLAIVGGGYTPSKYA VEGFNDSLRR DMKAFGVHVS CIEPGLFKTN LADPVKVIEK KLAIWEQLSP DIKQQYGEGY IEKSLDKLKG NKSYVNMDLSPVVECMDHAL TSLFPKTHYA AGKDAKIFWI PLSHMPAALQ DFLLLKQKAE LANPKAV
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Metabolic pathways; Retinol metabolism