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Recombinant Human 3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase, His-tagged

Cat. No.: HMGCL-1562H
Product Overview: Recombinant human HMGCL protein, fused to His-tag at N-terminus, was expressed in E.coli.
Description: HMGCL, also known as hydroxymethylglutaryl-CoA lyase, is a mitochondrial matrix protein that belongs to the HMG-CoA lyase family. It exists as a homodimer and participates in leucine catabolism and ketogenesis, the hepatic synthesis of ketone bodies that, during fasting, provide a major source of energy for heart, brain and kidney. More specifically, it catalyzes the final step of these processes, the cleavage of 3-hydroxy-3-methylglutaryl-CoA to acetoacetic acid and acetyl-CoA.
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea.
Molecular Weight: 34.2 kDa (323aa)
Purity: >85% by SDS-PAGE
Concentration: 1.0 mg/ml (determined by Bradford assay)
Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMTLPKR VKIVEVGPRD GLQNEKNIVS TPVKIKLIDM LSEAGLSVIE TTSFVSPKWV PQMGDHTEVLKGIQKFPGIN YPVLTPNLKG FEAAVAAGAK EVVIFGAASE LFTKKNINCS IEESFQRFDA ILKAAQSANI SVRGYVSCAL GCPYEGKISPAKVAEVTKKF YSMGCYEISL GDTIGVGTPG IMKDMLSAVM QEVPLAALAV HCHDTYGQAL ANTLMALQMG VSVVDSSVAG LGGCPYAQGASGNLATEDLV YMLEGLGIHT GVNLQKLLEA GNFICQALNR KTSSKVAQAT CKL
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways: Butanoate metabolism; Fatty acid; Formation of Acetoacetic Acid; Ketone body metabolism; Metabolic pathways; Metabolism of lipids and lipoproteins; Peroxisome; Synthesis and Degradation of Ketone Bodies; Synthesis of Ketone Bodies; Valine
Gene Name: HMGCL 3-hydroxymethyl-3-methylglutaryl-CoA lyase [ Homo sapiens ]
Official Symbol: HMGCL
Synonyms: HMGCL; 3-hydroxymethyl-3-methylglutaryl-CoA lyase; HL; hydroxymethylglutaryl-CoA lyase, mitochondrial; HMG-CoA lyase; OTTHUMP00000003005; OTTHUMP00000223529; OTTHUMP00000223530; EC 4.1.3.4; hydroxymethylglutaricaciduria; 3-hydroxy-3-methylglutaryl-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase; 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
Gene ID: 3155
mRNA Refseq: NM_000191
Protein Refseq: NP_000182
MIM: 613898
UniProt ID: P35914
Chromosome Location: 1p36.1-p35
Function: carboxylic acid binding; fatty-acyl-CoA binding; hydroxymethylglutaryl-CoA lyase activity; lyase activity; metal ion binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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