||Recombinant human PTPMT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||PTPMT1 (protein tyrosine phosphatase mitochondrial 1), also known as MOSP or PLIP (phosphoinositide lipid phosphatase) and previouslyknown as DUSP23, is a widely expressed PTP membrane protein with high expression levels in pancreatic beta cells. This protein exclusively localizes to the matrix face of the inner membrane of the mitochondrion. It is responsible for dephosphorylating mitochondrial proteins and therefore plays a significant role in the production of ATP and secretion of insulin. For its substrate, PTPMT1 displays a specific preference for the lipid signaling molecule phosphatidylinositol 5-phosphate.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15mM NaCl.
||22.6 kDa (199aa), confirmed by MALDI-TOF.
||>95% by SDS-PAGE
||1 mg/ml (determined by Bradford)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MGSHMKVPGR AHRDWYHRID PTVLLGALPL RSLTRQLVQD ENVRGVITMN EEYETRFLCN SSQEWKRLGV EQLRLSTVDM TGIPTLDNLQ KGVQFALKYQ SLGQCVYVHC KAGRSRSATM VAAYLIQVHK WSPEEAVRAI AKIRSYIHIR PGQLDVLKEF HKQITARATK DGTFVISKT
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.