Recombinant Human Interleukin 10, His-tagged
Cat.No. : | IL10-530H |
Product Overview : | Recombinant IL10 protein, fused to His-tag at N-terminus, was expressed in E. coli and was purified by conventional chromatography techniques. |
- Specification
- Gene Information
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Cat. No. : | IL10-530H |
Description : | Interleukin-10 (IL 10) is also known as human cytokine synthesis inhibitory factor (CSIF) and inhibits the synthesis of pro-inflammatory cytokines like IFN-gamma, IL2, IL3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. This protein has effects in immunoregulation and inflammation and down-regulates the expression of Th1 cytokines, MHC class II proteins, and costimulatory molecules on macrophages. |
Concentration : | 0.5 mg/ml |
Source : | Human |
Host : | E.coli |
Form : | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol. |
Purity : | > 95% by SDS - PAGE |
Sequence : | 19-178 amino acids: MGSSHHHHHH SSGLVPRGSH MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N |
Molecular Mass : | 20.94 kDa (181 aa), confirmed by MALDI-TOF. |
Applications : | SDS-PAGE |
Endotoxin Levels : | < 1.0 EU per ug of protein (determined by LAL method) |
Storage : | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
Gene Name : | IL10 interleukin 10 [ Homo sapiens ] |
Official Symbol : | IL10 |
Synonyms : | CSIF; IL10A; MGC1264 50; MGC126451; TGIF; B-TCGF; IL-10; Cytokine synthesis inhibitory factor; Interleukin-10 precursor; IL10; interleukin 10 |
Gene ID : | 3586 |
mRNA Refseq : | NM_000572 |
Protein Refseq : | NP_000563 |
MIM : | 124092 |
UniProt ID : | P22301 |
Chromosome Location : | 1q31-q32 |
Pathway : | Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; T cell receptor signaling pathway |
Function : | cytokine activity; interleukin-10 receptor binding; protein binding |
Products Types
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◆ Lysates | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionCutting-edge imaging techniques, alongside in vivo disease models, allow for precise assessment of therapeutic effects and tissue responses to IL-10-based treatments.
Advanced technologies, including single-cell RNA sequencing and flow cytometry, enable a granular analysis of IL-10's impact on diverse immune cell subpopulations.
Genotyping studies and genetic association analyses help correlate variations in IL-10 and its receptor with disease susceptibility across diverse populations.
Advanced immunomonitoring tools, including multiplex cytokine assays and immune cell phenotyping, provide comprehensive safety evaluations of IL-10 interventions in clinical trials.
In vitro models involve exposing immune cells to IL-10 and assessing changes in cytokine secretion and cell activation markers to uncover its anti-inflammatory mechanisms.
State-of-the-art techniques such as phosphoproteomics, mass spectrometry, and protein-protein interaction assays provide insights into the intricate signaling cascades activated by IL-10.
Dose-response studies in animal models coupled with outcome measures like cytokine profiles and immune cell phenotypes help determine the optimal IL-10 dosage and administration routes.
Experimental designs encompass co-stimulation experiments, coupled with functional assays and high-dimensional analysis, to elucidate synergistic effects involving IL-10 and other cytokines.
Techniques like RT-PCR, immunohistochemistry, and spatial transcriptomics are employed to characterize IL-10 receptor expression patterns in specific tissues and disease contexts.
CRISPR/Cas9 gene editing is used to create knockout or overexpression models, combined with functional assays, to decipher IL-10's regulatory roles in autoimmune disease pathways.
Customer Reviews (3)
Write a reviewEffective in anti-inflammatory assays.
High purity levels.
Stable under long-term storage.
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