Description : |
An IGFBP5 gene was recently identified as being important for adaptation to varying water salinity in fish. |
Source : |
E.coli |
Species : |
Human |
Form : |
Lyophilized from a 0.2μm filtered concentrated solution in 10 mM Sodium Citrate, pH 3.0. |
Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.4 μg/ml, corresponding to a specific activity of > 2500 IU/mg in the presence of 15 ng/ml of rHuIGF-II. |
Molecular Mass : |
Approximately 28.6 kDa, a single non-glycosylated polypeptide chain containing 252 amino acids. |
Protein length : |
252 |
AA Sequence : |
LGSFVHCEPCDEKALSMCPPSPLGC ELVKEPGCGCCMTCALAEGQSCGVY TERCAQGLRCLPRQDEEKPLHALLH GRGVCLNEKSYREQVKIERDSREHE EPTTSEMAEETYSPKIFRPKHTRIS ELKAEAVKKDRRKKLTQSKFVGGAE NTAHPRIISAPEMRQESEQGPCRRH MEASLQELKASPRMVPRAVYLPNCD RKGFYKRKQCKPSRGRKRGICWCVD KYGMKLPGMEYVDGDFQCHTFDSSN VE |
Endotoxin : |
Less than 0.1 EU/μg of rHuIGF-BP5 as determined by LAL method. |
Purity : |
>96% by SDS-PAGE and HPLC analysis. |
Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |