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Recombinant Human PTH protein

Cat.No. : PTH-03H
Product Overview : Recombinant Human PTH protein (7-34 a.a.) was expressed in Escherichia coli.
Description : Parathyroid hormone (PTH) is a single polypeptide of 84 amino acids. PTH is a critical hormone in the regulation of Ca2+ homeostasis. PTH is secreted by the parathyroid glands, which promote release of calcium from bone to extracellular fluid by activating osteoblasts and inhibiting osteoclasts, indirectly promote increased intestinal absorption of calcium, and promote renal tubular reabsorption of calcium and increased renal excretion of phosphates. It is a major regulator of bone metabolism. Secretion of parathyroid hormone increases when the level of calcium in the extracellular fluid is low. PTH (7-34), which is a PTH/PTHrP receptor antagonist, can stimulate hair growth and epidermal proliferation in mice.
Source : E.coli
Species : Human
Form : Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity : Fully biologically active when compared to standard. The specific activity is determined by UMR106 cell/cAMP method, corresponding to a specific activity of > 1.0 × 104 IU/mg.
Molecular Mass : Approximately 3.4 kDa, a single non-glycosylated polypeptide chain containing 28 amino acids.
Protein length : 28
AA Sequence : LMHNLGKHLNSMERVEWLRKKLQDVHNF
Endotoxin : Less than 1 EU/µg of rHuPTH7-34 as determined by LAL method.
Purity : >97% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : PTH
Official Symbol : PTH
Synonyms : PTH; parathyroid hormone; parathormone; parathyrin; parathyroid hormone 1; PTH1;
Gene ID : 5741
mRNA Refseq : NM_000315
Protein Refseq : NP_000306
MIM : 168450
UniProt ID : P01270

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