Recombinant HBV core protein CDA017

Recombinant HBV core protein

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Recombinant HBV core protein

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Cat.No. : CDA017
Product Overview : Recombinant HBV core (amino acids 1-183) was expressed in E. coli.
Description : Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA (pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.
Source : E. coli
Species : HBV
Form : 7.5mM phosphate buffer pH-7.2, 75mM NaCl and 50% glycerol.
Bio-activity : Immunoreactive with sera HBV-infected individuals.
Molecular Mass : 18 kDa recombinant protein contains the HBV core ayw immunodominant region,
AA Sequence : MDIDPYKEFG ASVELLSFLP SDFFPSIRDL LDTASALYRE ALESPEHCSPHHTALRQAIL CWGELMNLAT WVGSNLEDPA SRELVVSYVN VNMGLKFRQL LWFHVSCLTF GRETVLEYLV SFGVWIRTPP AYRPPNAPIL STLPETTVVR RRGRSPRRRT PSPRRRRSQS PRRRRSQSRE SQC.
Purity : HBV Core protein is >95% pure as determined by SDS-PAGE.
Storage : HBV Core protein although stable at 4 centigrade for 1 week, should be stored below -18 centigrade.Please prevent freeze thaw cycles.

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