|Cat. No. :
||IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two α and two β subunits. Both the α and β subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The α subunit of IGF-I receptor is extracellular while the β subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. A DNA sequence encoding the extracellular domain of human IGF-I receptor pre-propeptide (amino acid residues Met 1 - Asn 932) with ten histidine residues added at the carboxy terminus was expressed in HEK293 cell line.
|Amino-Acid Sequence :
|M. W. :
||Expressed in HEK293
||> 90%, as determined by SDS-PAGE and visualized by silver stain.
||Lyophilized from a 0.2 μm filtered solution in PBS containing 5% trehalose
||< 1.0 EU per 1 μg of the protein as determined by the LAL method.
||It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100 μg/mL.
||Lyophilized samples are stable for up to twelve months from date of receipt at -20° C to -70° C. Upon reconstitution, this cytokine can be stored under sterile conditions at -20° C to -70° C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.