Recombinant Human Tumor Necrosis Factor-Alpha Mutant

Cat. No.: TNF-38H
Product Overview: Recombinant Human Tumor Necrosis Factor-a Variant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 151 amino acids and having a molecular mass of 16598 Dalton. The TNF-alpha Variant is purified by standard chromatographic techniques.
Description: The clinical use of the potent anti-tumor activity of TNF-a has been limited by the proinflammatory side effects including fever, dose-limiting hypotension, hepatotoxicity, intravascular thrombosis, and hemorrhage. Designing clinically applicable TNF-a mutants with low systemic toxicity has been an intense pharmacological interest. Human TNF-α, which binds to the murine TNF-R55 but not to the mouse TNF-R75, exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-a, which binds to both murine TNF receptors. Based on these results, many TNF-α mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro, and exhibited lower systemic toxicity in vivo.
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation: The protein was lyophilized after extensive dialysis against 0.5 x PBS pH -7.
Solubility: It is recommended to reconstitute the lyophilized Interleikin-5 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Specific Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05 ng/ml, corresponding to a Specific Activity of 1 x 108 IU/mg.
Stability: Lyophilized Tumor Necrosis Factor-a Variant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a Variant should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name: TNF tumor necrosis factor (TNF superfamily, member 2) [ Homo sapiens ]
Synonyms: TNF; DIF; TNF-alpha; TNFSF2; TNFA; TNF-a; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; macrophage-derived; TNF superfamily, member 2; Tumor necrosis factor ligand superfamily member 2; OTTHUMP00000029281; OTTHUMP00000037669
Gene ID: 7124
mRNA Refseq: NM_000594
Protein Refseq: NP_000585
MIM: 191160
UniProt ID: P01375
Chromosome Location: 6p21.3
Function: cytokine activity; identical protein binding; tumor necrosis factor receptor binding

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