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Recombinant Mouse Chemokine (C-X-C Motif) Ligand 2

Cat. No.: Cxcl2-38M
Product Overview: Recombinant Mouse GRO-Beta also caled mouse MIP-2 produced inE.Coliis a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7849 Dalton. The GRO-Beta is purified by proprietary chromatographic techniques.
Description: Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2). CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2.
Source: Escherichia Coli.
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Val-Ala-Ser.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation: The protein was lyophilized with no additives.
Solubility: It is recommended to reconstitute the lyophilized GRO-beta Mouse in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The Biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with 5 ng/ml of recombinant mouse MIP-2.
Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.015 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of GRO-as a Reference Standard.
Storage: Lyophilized GRO-although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL2 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name: Cxcl2 chemokine (C-X-C motif) ligand 2 [ Mus musculus ]
Synonyms: Cxcl2; chemokine (C-X-C motif) ligand 2; GROb; Gro2; Mip2; Scyb; MIP-2; Scyb2; MIP-2a; Mgsa-b; CINC-2a; small inducible cytokine subfamily, member 2; MIP2
Gene ID: 20310
mRNA Refseq: NM_009140
Protein Refseq: NP_033166
UniProt ID: P10889
Chromosome Location: 5 E1; 5 51.0 cM
Pathway: Chemokine signaling pathway; Cytokine-cytokine receptor interaction
Function: chemokine activity; cytokine activity

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