| Cat. No.: | TNFSF11-117H |
| Product Overview: | Recombinant Human sRANKL produced inE.Coliis a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 20006 Dalton. CD254 is purified by proprietary chromatographic techniques. |
| Description: | RANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. |
| Source: | Escherichia Coli. |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity: | Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Formulation: | The protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM Tris, pH-7.6 and 50mM sodium chloride. |
| Solubility: | It is recommended to reconstitute the lyophilized sRANKL in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Lys-Ala-Met. |
| Biological Activity: | The activity is determined by its ability to induce osteoclast formation in RAW264.7 cells using a conc. of 5.0-10.0 ng/ml and by dose dependant stimulation of IL-8 production in human PBMC which was found to be <10ng/ml. |
| Storage: | Lyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Gene Name: | TNFSF11 tumor necrosis factor (ligand) superfamily, member 11 [ Homo sapiens ] |
| Synonyms: | TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TRANCE; hRANKL2; tumor necrosis factor ligand superfamily, member 11; OTTHUMP00000178585;osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; Tumor necrosis factor ligand superfamily member 11, membrane form; Tumor necrosis factor ligand superfamily member 11, soluble form; CD254 antigen; Osteoprotegerin ligand |
| Gene ID: | 8600 |
| mRNA Refseq: | NM_003701 |
| Protein Refseq: | NP_003692 |
| MIM: | 602642 |
| UniProt ID: | O14788 |
| Chromosome Location: | 13q14 |
| Pathway: | Cytokine-cytokine receptor interaction |
| Function: | cytokine activity; protein binding; receptor activity; tumor necrosis factor receptor binding |
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
Download Datasheet
See All Tnfsf11 Products
Download Datasheet