Cat. No.: |
TNFSF11-117H |
Product Overview: |
Recombinant Human sRANKL produced inE.Coliis a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 20006 Dalton. CD254 is purified by proprietary chromatographic techniques. |
Description: |
RANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. |
Source: |
Escherichia Coli. |
Physical Appearance: |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity: |
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation: |
The protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM Tris, pH-7.6 and 50mM sodium chloride. |
Solubility: |
It is recommended to reconstitute the lyophilized sRANKL in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Amino Acid Sequence: |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Lys-Ala-Met. |
Biological Activity: |
The activity is determined by its ability to induce osteoclast formation in RAW264.7 cells using a conc. of 5.0-10.0 ng/ml and by dose dependant stimulation of IL-8 production in human PBMC which was found to be <10ng/ml. |
Storage: |
Lyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |