Active Recombinant Human ITGAVB3

• Cat.No.: ITGAVB3-103H
• Product Overview: Recombinant Human ITGAVB3 protein (Human ITGAV & ITGB3) Phe31–Val992 (Integrin αV) and Gly27–Asp718(Integrin β3 ) was co-expressed in CHO Cells.

Specification

• Cat. No.: ITGAVB3-103H
• Description: ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; ''ITGB1'', ''ITGB3'', ''ITGB5'', ''ITGB6'', and ''ITGB8''), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. Alternative splicing results in multiple transcript variants.
• Source: CHO Cells
• Molecular Mass: 110.5 kDa (αV subunit), 80.8 kDa (β3 subunit)
• Predicted N terminal: Phe31 & Gly27
• Formulation: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CaCl2. See Certificate of Analysis for details.
• Purity: >90%, by SDSPAGE under reducing conditions and visualized by silver stain.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Vitronectin at 1 μg/mL can bind. Recombinant Human Integrin αVβ3 with an apparent KD < 10 nM.
• EndotoxinLevel: <0.10 EU per 1 μg of the protein by the LAL method.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Storage: 12 months from date of receipt, ­20 to ­70 °C as supplied. Avoid repeated freeze­thaw cycles.
• OfficialSymbol: ITGAV

Gene Information

• Gene Name: ITGAV integrin, alpha V [ Homo sapiens ]
• Synonyms: ITGAV; integrin, alpha V; antigen identified by monoclonal L230; MSK8; VNRA; integrin alpha-V; CD51; VTNR; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); Vitronectin receptor subunit alpha; vitronectin receptor; integrin alphaVbeta3; CD51 antigen; antigen identified by monoclonal antibody L230
• Gene ID: 3685
• mRNA Refseq: NM_002210
• Protein Refseq: NP_002201
• MIM: 193210
• UniProt ID: P06756
• Chromosome Location: 2q31-q32
• Pathway: Adaptive Immune System; Antigen processing-Cross presentation; Arrhythmogenic right ventricular cardiomyopathy (ARVC); Axon guidance
• Function: contributes_to insulin-like growth factor I binding; contributes_to opsonin binding; protein binding; contributes_to protein kinase C alpha binding; receptor activity