Active Recombinant Human GTF2E2, His-tagged

• Cat.No.: GTF2E2-8456H
• Product Overview: Recombinant Human GTF2E2, fused with His tag, was expressed in E. coli.

Specification

• Description: The human Transcription Factor IIE (TFIIE) is composed of 56 kDa (a) and 34 kDa (?) subunits and is shown to be a heterotetramer . The 56-kDa subunit contains a region similar to a zinc-binding domain and a region sharing homology with the catalytic loop of a kinase domain. TFIIE binds to RNA polymerase II in solution and joins the pre-initiation complex probably concomitant with RNA polymerase II and TFIIF.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
• Bio-activity: 1 unit equals 1 ng of purified protein. 20 units are sufficient for an in vitro reconstituted transcription assay in the presence of 56-kDa subunit; 100 units are sufficient for a protein-protein interaction assay.
• AA Sequence: MDPSLLRERE LFKKRALSTP VVEKRSASSE SSSSSSKKKK TKVEHGGSSG SKQNSDHSNG SFNLKALSGS SGYKFGVLAK IVNYMKTRHQ RGDTHPLTLD EILDETQHLD IGLKQKQWLM TEALVNNPKI EVIDGKYAFK PKYNVRDKKA LLRLLDQHDQ RGLGGILLED IEEALPNSQK AVKALGDQIL FVNRPDKKKI LFFNDKSCQF SVDEEFQKLW RSVTVDSMDE EKIEEYLKRQ GISSMQESGP KKVAPIQRRK KPASQKKRRF KTHNEHLAGV LKDYSDITSS K
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Concentration: 250 μg/ml
• Shipping: Dry Ice

Gene Information

• Gene Name: GTF2E2 general transcription factor IIE, polypeptide 2, beta 34kDa [ Homo sapiens ]
• Official Symbol: GTF2E2
• Synonyms: GTF2E2; general transcription factor IIE, polypeptide 2, beta 34kDa; general transcription factor IIE, polypeptide 2 (beta subunit, 34kD); transcription initiation factor IIE subunit beta; FE; TF2E2; TFIIE B; TFIIE-beta; general transcription factor IIE subunit 2; TFIIE-B
• Gene ID: 2961
• mRNA Refseq: NM_002095
• Protein Refseq: NP_002086
• MIM: 189964
• UniProt ID: P29084
• Chromosome Location: 8p12
• Pathway: Basal transcription factors, organism-specific biosystem; Basal transcription factors, conserved biosystem; Disease, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; Gene Expression, organism-specific biosystem; HIV Infection, organism-specific biosystem; HIV Life Cycle, organism-specific biosystem
• Function: DNA binding; protein binding