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Recombinant Human Interferon, Beta 1, Fibroblast

Cat. No. : IFNB1-19H
Product Overview : Recombinant Human Interferon-beta 1a produced inCHO (Chinese Hamster Ovarian) cellsis a single, glycosylated polypeptide chain containing 166 amino acids and having a molecular mass of 22500 Dalton. IFN-beta1a is purified by proprietary chromatographic techniques.
Description : Interferon-beta 1b has antiviral, antibacterial and anticancer activities.
Source : CHO (Chinese Hamster Ovarian) cells
Amino Acid Sequence : The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu.
Physical Appearance : Lyophilized from 1mg/ml solution containing 91 mg Human Albumin, and 620mg mannitol, and 9 mg Sodium Acetate.
Purity : Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation : Lyophilized from a concentrated 1mg/ml solution containing PBS 7.4.
Solubility : It is recommended to reconstitute the lyophilized Interferon beta 1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity : The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 270 x 106 IU/mg.
Storage : Lyophilized Interferon-beta 1a although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IFN-Beta1a should be stored at 4℃ between 2-7 days and for future use below -18℃.Please prevent freeze-thaw cycles.
Gene Name : IFNB1 interferon, beta 1, fibroblast [ Homo sapiens ]
Synonyms : IFNB1; Interferon, beta 1, fibroblast; IFB; IFF; IFNB; MGC96956; Interferon beta; Leukocyte interferon; B cell interferon; Type I interferon; IFNB1; IFN-b 1a; IFN-beta; OTTHUMP00000021131; Fibroblast interferon
Gene ID : 3456
mRNA Refseq : NM_002176
Protein Refseq : NP_002167
MIM : 147640
UniProt ID : P01574
Chromosome Location : 9p21
Pathway : Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; RIG-I-like receptor signaling pathway; Toll-like receptor signaling pathway
Function : cytokine activity; interferon-alpha/beta receptor binding; transcription corepressor activity

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