| Description : |
C1 Inhibitor is a member of the serpin family of structurally related proteins, and is the primary regulator of the immune complement system. C1Inhibitor is a protease inhibitor that functions to inhibit the complement system in order to prevent over-activation or spontaneous activation. Inhibition is achieved by binding to and irreversibly inhibiting the C1r and C1s proteases of the C1 complex, which has the effect of shutting down all subsequent downstream events in the complement activation cascade. C1inhibitor can also inhibit various other proteases, including Kallikrein, Factor XIa, and Factor XIIa. Deficiencies in C1inhibitor are the primary cause of hereditary angioedema (HAE, hereditary angioneurotic edema), a disease characterized by edema in the respiratory and gastrointestinal tracts. In certain clinical situations, the direct administration of C1inhibitor can be used to treat HAE and certain other conditions. |
| Source : |
CHO |
| Species : |
Human |
| Form : |
Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodiun Phosphate, PH 7.5. |
| Bio-activity : |
Measured by its ability to inhibit recombinant human complement component C1a cleavage of a colorimetric peptide substrate, N Carbobenzyloxy-Lys-ThioBenzyl ester (Z-K-SBzl). The expected IC50 is ≤ 2.6 nM. |
| Molecular Mass : |
Is a highly glycosylated glycoprotein containing 445 amino acid residues (49.4kDa). Glycosylated C1 Inhibitor migrates at an apparent molecular weight of approximately 80-90 kDa by SDS PAGE analysis under reducing conditions. |
| AA Sequence : |
VEPILEVSS LPTTNSTTN SATKITANT TDEPTTQPT TEPTTQPTI QPTQPTTQL PTDSPTQPT TGSFCPGPV TLCSDLESH STEAVLGDA LVDFSLKLY HAFSAMKKV ETNMAFSPF SIASLLTQV LLGAGENTK TNLESILSY PKDFTCVHQ ALKGFTTKG VTSVSQIFH SPDLAIRDT FVNASRTLY SSSPRVLSN NSDANLELI NTWVAKNTN NKISRLLDS LPSDTRLVL LNAIYLSAK WKTTFDPKK TRMEPFHFK NSVIKVPMM NSKKYPVAH FIDQTLKAK VGQLQLSHN LSLVILVPQ NLKHRLEDM EQALSPSVF KAIMEKLEM SKFQPTLLT LPRIKVTTS QDMLSIMEK LEFFDFSYD LNLCGLTED PDLQVSAMQ HQTVLELTE TGVEAAAAS AISVARTLL VFEVQQPFL FVLWDQQHK FPVFMGRVY DPRA |
| Endotoxin : |
Endotoxin level is <0.10 ng/ug of the protein(< 1 EU/ug). |
| Purity : |
Above 95% by SDS-PAGE gel and HPLC analyses. |
| Storage : |
Store at -20 centigrade. |
| Reconstitution : |
Centifuge the viar prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to furthur dilute in a buffer containing a carrier protein(example 0.1% BSA) and store in working aliquots at -20 centigrade to -80 centigrade. |
