||Recombinant Human PEDF produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 413 amino acids and having a molecular mass of 46.1 kDa. The Human Sentrin-F1 is fused to His tag at N-Terminus. The Human PEDF is purified by proprietary chromatographic techniques.
||PEDF is a neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. SerpinF1 is a potent inhibitor of angiogenesis. EPC1 doesn't undergo the stressed to relaxed conformation transition characteristic as of the active serpins since it exhibits no serine protease inhibitory activity. Aqueous humour level of asymmetric dimethylarginine is correlated with PEDF in humans. ADMA and PEDF levels are increased in response to inflammation in uveitis. Lack of PEDF expression is a potent factor for the enhancement of tumor growth and angiogenesis in breast cancer. PEDF & VEGF genes contribute to the development of diabetic retinopathy. PEDF and VEGF structural changes in blood vessel wall play an important role in the pathophysiology of PD patients. VEGF secreted by retinal pigment epithelial cells upregulates PEDF expression via VEGFR-1 in an autocrine manner. Sentrin-F1 concentration in the aqueous humor of diabetic patients predicts who will develop progression of retinopathy. PEDF blocks angiogenic effects of leptin through its anti-oxidative properties.
|Amino Acid Sequence:
||MRGSHHHHHH GMASQNPASP PEEGSPDPDS TGALVEEEDP FFKVPVNKLA AAVSNFGYDL YRVRSSMSPT TNVLLSPLSV ATALSALSLG AEQRTESIIH RALYYDLISS PDIHGTYKEL LDTVTARQKN LKSASRIVFE KKLRIKSSFV APLEKSYGTR PRVLTGNPRL DLQEINNWVQ AQMKGKLARS TKEIPDEISI LLLGVAHFKG QWVTKFDSRK TSLEDFYLDE ERTVRVPMMS DPKAVLRYGL DSDLSCKIAQ LPLTGSMSII FFLPLKVTQN LTLIEESLTS EFIHDIDREL KTVQAVLTVP KLKLSYEGEV TKSLQEMKLQ SLFDSPDFSK ITGKPIKLTQ VEHRAGFEWN EDGAGTTPSP GLQPAHLTFP LDYHLNQPFI FVLRDTDTGA LLFIGKILDP RGP
||Sterile Filtered White lyophilized (freeze-dried) powder.
||Greater than 95% as determined by SDS-PAGE.
||The sterile filtered concentrated (0.5mg/ml) protein solution was lyophilized with 20mM Tris & 150mM NaCl pH-7.5.
||Add Add deionized water to a working concentration of 0.5 mg/ml and let the lyophilized pellet dissolve completely.
||Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.