Recombinant Human EIF2S1, GST-tagged

• Cat.No.: EIF2S1-12361H
• Product Overview: Recombinant Human EIF2S1 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Description: The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).
• Source: E.coli
• Species: Human
• Tag: GST
• Protein length: 1-315a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa [ Homo sapiens ]
• Official Symbol: EIF2S1
• Synonyms: EIF2S1; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; EIF2, eukaryotic translation initiation factor 2, subunit 1 (alpha, 35kD ); eukaryotic translation initiation factor 2 subunit 1; EIF 2alpha; EIF2A; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha; EIF2; EIF-2; EIF-2A; EIF-2alpha
• Gene ID: 1965
• mRNA Refseq: NM_004094
• Protein Refseq: NP_004085
• MIM: 603907
• UniProt ID: P05198
• Chromosome Location: 14q21.3
• Pathway: Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem; Cap-dependent Translation Initiation, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; Formation of the ternary complex, and subsequently, the 43S complex, organism-specific biosystem; GTP hydrolysis and joining of the 60S ribosomal subunit, organism-specific biosystem
• Function: RNA binding; protein binding; ribosome binding; translation initiation factor activity