Recombinant Human IARS2 protein, His-tagged

• Cat.No.: IARS2-14027H
• Product Overview: Recombinant Human IARS2 protein(425-773 aa), fused with His tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein Length: 425-773 aa
• AA Sequence: KMSKSLGNVIHPDVVVNGGQDQSKE PPYGADVLRWWVADSNVFTEVAIGP SVLNAARDDISKLRNTLRFLLGNVA DFNPETDSIPVNDMYVIDQYMLHLL QDLANKITELYKQYDFGKVVRLLRT FYTRELSNFYFSIIKDRLYCEKEND PKRRSCQTALVEILDVIVRSFAPIL PHLAEEVFQHIPYIKEPKSVFRTGW ISTSSIWKKPGLEEAVESACAMRDS FLGSIPGKNAAEYKVITVIEPGLLF EIIEMLQSEETSSTSQLNELMMASE STLLAQEPREMTADVIELKGKFLIN LEGGDIREESSYKVIVMPTTKEKCP RCWKYTAESSDTLCPRCAEVVSGK
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: IARS2 isoleucyl-tRNA synthetase 2, mitochondrial [ Homo sapiens ]
• Official Symbol: IARS2
• Synonyms: IARS2; isoleucyl-tRNA synthetase 2, mitochondrial; isoleucine--tRNA ligase, mitochondrial; FLJ10326; isoleucine tRNA ligase 2; mitochondrial; isoleucine tRNA ligase 2, mitochondrial; isoleucyl-tRNA synthetase, mitochondrial; mitochondrial isoleucine tRNA synthetase; isoleucine-tRNA synthetase 2, mitochondrial; ILERS
• Gene ID: 55699
• mRNA Refseq: NM_018060
• Protein Refseq: NP_060530
• MIM: 612801
• UniProt ID: Q9NSE4