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Recombinant human ECI1, His-tagged

Cat.No. : ECI1-2445H
Product Overview : Recombinant human ECI1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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Description : ECI1 is a member of the hydratase/isomerase superfamily. The protein is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described.
Source : E.coli
Species : Human
Tag : His
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Molecular Mass : 31.1 kDa (284aa), confirmed by MALDI-TOF
AA Sequence : MGSSHHHHHH SSGLVPRGSH MGSFGSQRVL VEPDAGAGVA VMKFKNPPVN SLSLEFLTEL VISLEKLEND KSFRGVILTS DRPGVFSAGL DLTEMCGRSP AHYAGYWKAV QELWLRLYQS NLVLVSAING ACPAGGCLVA LTCDYRILAD NPRYCIGLNE TQLGIIAPFW LKDTLENTIG HRAAERALQL GLLFPPAEAL QVGIVDQVVP EEQVQSTALS AIAQWMAIPD HARQLTKAMM RKATASRLVT QRDADVQNFV SFISKDSIQK SLQMYLERLK EEKG
Purity : >90% by SDS - PAGE
Applications : SDS-PAGE
Storage : Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration : 0.25 mg/ml (determined by Bradford assay)
Gene Name : ECI1 enoyl-CoA delta isomerase 1 [ Homo sapiens ]
Official Symbol : ECI1
Synonyms : ECI1; enoyl-CoA delta isomerase 1; DCI, dodecenoyl CoA isomerase , dodecenoyl Coenzyme A delta isomerase (3,2 trans enoyl Coenzyme A isomerase); enoyl-CoA delta isomerase 1, mitochondrial; 3; 2 trans enoyl CoA isomerase; dodecenoyl-CoA isomerase; D3,D2-enoyl-CoA isomerase; acetylene-allene isomerase; 3,2 trans-enoyl-CoA isomerase; delta3, delta2-enoyl-CoA isomerase; 3,2 trans-enoyl-Coenzyme A isomerase; delta(3),Delta(2)-enoyl-CoA isomerase; 3,2-trans-enoyl-CoA isomerase, mitochondrial; dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase); dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase); DCI;
Gene ID : 1632
mRNA Refseq : NM_001178029
Protein Refseq : NP_001171500
MIM : 600305
UniProt ID : P42126
Chromosome Location : 16p13.3
Pathway : Fatty Acid Beta Oxidation, organism-specific biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Mitochondrial Fatty Acid Beta-Oxidation, organism-specific biosystem;
Function : dodecenoyl-CoA delta-isomerase activity; dodecenoyl-CoA delta-isomerase activity; identical protein binding; intramolecular oxidoreductase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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