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Recombinant YFP

Cat.No.: YFP-101
Product Overview: The recombinant YFP is expressed and purified from transformed E. coli using a method that ensures high purity and maximal YFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The protein is a 26.4 kDa monomer with 238 amino acids, Ex./Em. = 525/538 nm, extinction coefficient 27,765 (at 280 nm).
Description: Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm.
Source: E.Coli
Form: Lyophilized
Molecular Mass: 26.4 kDa
AA Sequence: MRGSGSSGAL LFHGKIPYVV EMEGNVDGHT FSIRGKGYGD ASVGKVDAQF ICTTGDVPVP WSTLVTTLTY GAQCFAKYGP ELKDFYKSCM PDGYVQERTI TFEGDGNFKT RAEVTFENGS VYNRVKLNGQ GFKKDGHVLG KNLEFNFTPH CLYIWGDQAN HGLKSAFKIC HEITGSKGDF IVADHTQMNT PIGGGPVHVP EYHHMSYHVK LSKDVTDHRD NMSLKETVRA VDCRKTYL
Endotoxin: ≤0.1 ng/µg of YFP
Purity: ≥97% by SDS-PAGE and HPLC
Applications: The protein is suitable as control reagent for YFP expression studies or as labeling reagent. Applications include: Use as standards for SDS-PAGE, Western blot analysis of YFP transfected cells, label other proteins, calibration of fluorometers and flow cytometers, fluorescence microscope, or microinjection of YFP into cells and tissues, etc. The recombinant YFP is ideal for fusion tag applications and is perfect for triple labeling with EGFP and RFP.
Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
Reconstitution: Centrifuge vial prior to opening. Add sterile distilled water to the vial to fully solubilize the protein to a concentration of 1.0 mg/ml.

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