Active Recombinant Human Inhibin Beta A chain, His tagged, Animal Free

• Cat.No.: INHBA-145H
• Product Overview: Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants and is purified by standard protien purification methods. Recombinant Human Inhibin, Beta A is a polypeptide chain containing 116 amino acids (311–426 ) and a His-tag at the N-terminal end. This product contains no animal-derived components or impurities. Animal Free product.

Specification

• Description: Inhibins are homodimers or heterodimers of the various β subunit isoforms, belonging to the TGFβfamily. Mature Inhibin Beta A has two 116 amino acids residues βA subunits (βA-βA). Inhibin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Inhibin plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells known to express Inhibin Beta A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydigcells, Sertolicells, andovarian granulosa cells. As with other members of the super-family, Inhibins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Inhibin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Inhibin type 2 receptors, ACVR2A, ACVR2B. The biological activity of Inhibin Beta A can be neutralized by inhibins and by the diffusible TGF-B antagonist, Follistatin.
• Source: Nicotiana benthamiana
• Species: Human
• Tag: His
• Form: Lyophilized from a Tris HCl 0.05M buffer at pH 7.4
• Bio-activity: Biological Activity:1. Effect of rh Activin A on mouse plasmacytoma cell line (MPC-11) cells proliferationThe biological activity of Activin A is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation. Cell proliferation was measured by MTT method. ED50 ≤ 10ng/ml.2. Effect of rh Activin A on keratinocytes differentiation.
• Molecular Mass: Several forms are observed with different predicted molecular masses such as monomer (13.7 kDa), dimmer ( 27.4 kDa) and multimeric forms.
• AA Sequence: HHHHHHGLECDGKVNICCKKQFFVS FKDIGWNDWIIAPSGYHANYCEGEC PSHIAGTSGSSLSFHSTVINHYRMR GHSPFANLKSCCVPTKLRPMSMLYY DDGQNIIKKDIQNMIVEECGCS
• Endotoxin: < 0.04 eu/μg protein (lal>
• Purity: >97% by SDS-PAGE gel
• Applications: Cell culture. Western Blot. Immunogen. Dermocosmetics (Inhibin Beta A increases the expression of E-cadherin and improves the distribution of e-cadherin in cell-cell contact areas).
• Stability: This lyophilized preparation is stable at 2-8oC for short term, long storage it should be kept at -20oC.
• Storage: Reconstituted protein should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended.
• Reconstitution: Lyophilized protein should be reconstituted in water following instructions of batch Quality Control sheet. At higher concentrations the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application.

Gene Information

• Gene Name: INHBA inhibin, beta A [ Homo sapiens ]
• Official Symbol: INHBA
• Synonyms: INHBA; inhibin, beta A; inhibin, beta A (activin A, activin AB alpha polypeptide); inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; FSH-releasing protein; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; EDF; FRP
• Gene ID: 3624
• mRNA Refseq: NM_002192
• Protein Refseq: NP_002183
• MIM: 147290
• UniProt ID: P08476
• Chromosome Location: 7p15-p13
• Pathway: ALK1 signaling events, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Glycoprotein hormones, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Peptide hormone biosynthesis, organism-specific biosystem
• Function: cytokine activity; follistatin binding; growth factor activity; hormone activity; identical protein binding; protein binding; protein heterodimerization activity; type II activin receptor binding