Recombinant Human GPD1 protein, His-tagged

• Cat.No.: GPD1-281H
• Product Overview: Recombinant Human GPD1 (Met1-Met349) protein was fused to His-tag at N-terminus and expressed in E.coli.

Specification

• Description: Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (GPD1), a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family, is also known as GPD-C or GPDH-C. The function of GPD1 is inhibited by zinc ions and sulfate. GPD1 can also interact selectively and non-covalently with nicotinamide adenine dinucleotide (NAD), a coenzyme involved in many redox and biosynthetic reactions; binding may be to either the oxidized form, NAD+, or the reduced form, NADH. Mutations in GPD1 gene are a cause of Hypertriglyceridemia, transient infantile (HTGIT).
• Source: E.coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met1
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 38.4 kDa. The protein migrates as 34 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: Met1-Met349
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: GPD1
• Official Symbol: GPD1
• Synonyms: GPD1; glycerol-3-phosphate dehydrogenase 1 (soluble); glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic; glycerophosphate dehydrogenase; glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic; GPD-C; HTGTI; GPDH-C; FLJ26652
• Gene ID: 2819
• mRNA Refseq: NM_001257199
• Protein Refseq: NP_001244128
• MIM: 138420
• UniProt ID: P21695