Active Recombinant Canine NTRK1, His tagged

• Cat.No.: NTRK1-295C
• Product Overview: Recombinant Canine NTRK1 (XP_851619.3) (Pro285-Lys410), fused with a N-terminal polyhistidine tag, was produced in Human Cells.

Specification

• Source: HEK293
• Species: Canine
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Measured by its ability to inhibit NGF-induced proliferation of TF‑1 human erythroleukemic cells.The ED50 for this effect is typically 0.4-1.6 µg/mL in the presence of 10 ng/mL of recombinant human NGF.
• Molecular Mass: The recombinant canine NTRK1 comprises 146 amino acids and has a predicted molecular mass of 16.2 kDa. The apparent molecular mass of the protein is approximately 27-33 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein Length: Pro285-Lys410
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.