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Active Recombinant Cynomolgus/Rhesus CD274 protein(Met1-Thr239), Fc-tagged

Cat.No. : CD274-131C
Product Overview : Recombinant Cynomolgus / rhesus CD274 (XP_015292694.1) (Met1-Thr239) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus. Cynomolgus and Rhesus CD274 sequences are identical.
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Source : HEK293
Species : Cynomolgus/Rhesus
Tag : C-Fc
Protein length : Met1-Thr239
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized human PD1-His at 10 μg/ml (100 μl/well) can bind Cynomolgus CD274-Fc, The EC50 of Cynomolgus CD274-Fc is 0.5-1.3 μg/ml.
Molecular Mass : The recombinant Cynomolgus / rhesus CD274 comprises 462 amino acids and has a calculated molecular mass of 52.3 KDa.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 95 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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