Active Recombinant Human IL-4

• Cat.No.: IL4-504H
• Product Overview: Recombinant Human IL-4 /Interleukin-4 Protein, HEK293 expressed(rhIL-4), His25-Ser153 (Accession# AAH67514)was expressed in human 293 cells (HEK293).

Specification

• Description: Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. Recently, researcher found that the cytokine IL-4 plays a key role in development of innate CD8+ T cells in the thymus of several gene-deficient mouse strains, including Itk, KLF2, CBP and Id3, without previous exposure to antigen.
• Source: HEK293
• Species: Human
• Predicted N Terminal: His25
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM MES, 200 mM NaCl, pH6.0. Normally Mannitol or Trehalose are added as protectants. before lyophilization.
• Bio-activity: The bio-activity was determined by dose-dependent stimulation of the proliferation of TF-1 cells. The ED50<0.2ng l, corresponding to a specific activity of>5X10^6 Unit/mg.
• Molecular Mass: rhIL-4 contains no "tag" and has a calculated MW of 14.9 kDa. DTT-reduced protein migrates as 15 and 19 kDa protein due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the or rhIL-4 by the LAL method.
• Purity: >95% purity as determined by SDS-PAGE of reduced rhIL-4.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade-8 centigrade); After reconstitution under sterile conditions for 1 month (4 centigrade-8 centigrade) or 3 months (-20 centigrade to -70 centigrade).

Gene Information

• Gene Name: IL4 interleukin 4 [ Homo sapiens ]
• Official Symbol: IL4
• Synonyms: IL4; interleukin 4; interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; BCGF 1; BCGF1; BSF1; IL 4; lymphocyte stimulatory factor 1; MGC79402; binetrakin; pitrakinra; IL-4; BSF-1; BCGF-1
• Gene ID: 3565
• mRNA Refseq: NM_000589
• Protein Refseq: NP_000580
• MIM: 147780
• UniProt ID: P05112
• Chromosome Location: 5q23-q31
• Pathway: Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD40/CD40L signaling, organism-specific biosystem
• Function: cytokine activity; growth factor activity; interleukin-4 receptor binding; protein binding