Recombinant Human RAD51D, His-tagged

• Cat.No.: RAD51D-5049H
• Product Overview: Recombinant human RAD51D protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: RAD51D is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, which are known to be involved in the homologous recombination and repair of DNA. This protein forms a complex with several other members of the RAD51 family, including RAD51L1, RAD51L2, and XRCC2. The protein complex formed with this protein has been shown to catalyze homologous pairing between single- and double-stranded DNA, and is thought to play a role in the early stage of recombinational repair of DNA.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 37.4 kDa (351aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGVLRVG LCPGLTEEMI QLLRSHRIKT VVDLVSADLE EVAQKCGLSY KALVALRRVL LAQFSAFPVN GADLYEELKT STAILSTGIG SLDKLLDAGL YTGEVTEIVG GPGSGKTQVC LCMAANVAHG LQQNVLYVDS NGGLTASRLL QLLQAKTQDE EEQAEALRRI QVVHAFDIFQ MLDVLQELRG TVAQQVTGSS GTVKVVVVDS VTAVVSPLLG GQQREGLALM MQLARELKTL ARDLGMAVVV TNHITRDRDS GRLKPALGRS WSFVPSTRIL LDTIEGAGAS GGRRMACLAK SSRQPTGFQE MVDIGTWGTS EQSATLQGDQ T
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RAD51D RAD51 homolog D (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: RAD51D
• Synonyms: RAD51D; RAD51 homolog D (S. cerevisiae); RAD51 (S. cerevisiae) like 3 , RAD51 like 3 (S. cerevisiae) , RAD51L3; DNA repair protein RAD51 homolog 4; HsTRAD; R51H3; recombination repair protein; Trad; RAD51-like protein 3; TRAD; BROVCA4; RAD51L3
• Gene ID: 5892
• mRNA Refseq: NM_001142571
• Protein Refseq: NP_001136043
• MIM: 602954
• UniProt ID: O75771
• Chromosome Location: 17q11
• Pathway: Homologous recombination, organism-specific biosystem; Homologous recombination, conserved biosystem
• Function: ATP binding; DNA binding; DNA-dependent ATPase activity; nucleoside-triphosphatase activity; nucleotide binding; protein binding