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Recombinant Mouse Il1f6 protein

Cat.No. : Il1f6-551M
Product Overview : Recombinant Mouse Il1f6 protein (153 a.a.) was expressed in Escherichia coli.
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  • Gene Information
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Description : Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36α is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. It is expressed by monocytes, B and T cells. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. Recombinant murine interleukin-36 alpha contains 153 amino acids residues which is a single non-glycosylated polypeptide. Specifically, mouse IL-36α shares 83 % a.a. sequence identity with rat IL-36α, 54 – 60 % with human, rabbit, equine and bovine IL-36α.
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered concentrated solution in PBS, 1 mM DTT, 3 % trehalose.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 25 ng/ml, corresponding to a specific activity of > 4.0 × 10⁴ IU/mg.
Molecular Mass : Approximately 17.1kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Protein length : 153
AA Sequence : RAASPSLRHVQDLSSRVWILQNNIL TAVPRKEQTVPVTITLLPCQYLDTL ETNRGDPTYMGVQRPMSCLFCTKDG EQPVLQLGEGNIMEMYNKKEPVKAS LFYHKKSGTTSTFESAAFPGWFIAV CSKGSCPLILTQELGEIFITDFEMI VVH
Endotoxin : Less than 1 EU/μg of rMuIL-36α, 153a.a. as determined by LAL method.
Purity : >95% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : Il1f6
Official Symbol : Il1f6
Synonyms : IL1F6; interleukin 1 family, member 6; interleukin-36 alpha; IL-1F6; Fil1epsilon; FIL1 epsilon; IL-1 epsilon; interleukin-1 epsilon; interleukin-1 homolog 1; interleukin-1 family member 6; interleukin 1 family, member 9; interleukin 1 family, member 6 (epsilon); Fil1; Il1f9; Il36a; IL-1H1; IL1RP2; MGC151479; MGC151481;
Gene ID : 54448
mRNA Refseq : NM_019450
Protein Refseq : NP_062323
UniProt ID : Q9JLA2

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Does IL36A carry a fever risk? 04/27/2022

In general, the use of IL36A does not cause significant fever reactions, but individual differences exist, if there are abnormalities, please consult a doctor.

How to prepare IL36A? 09/07/2021

The IL36A is expressed, purified, and constructed using recombinant DNA technology in a suitable host expression system, usually through genetic engineering.

In what inflammatory diseases does recombinant IL36A have potential applications? 05/24/2021

IL36A has potential application value in psoriasis, arthritis and other inflammatory diseases, but the specific efficacy needs further study.

Does IL36A have anti-tumor effects? 08/22/2020

IL36A has been shown in some studies to have anti-proliferative and anti-invasive effects on certain tumors, but more studies are needed to confirm its specific mechanism of action and effect.

How is the research progress of IL36A? 06/05/2020

The research of IL36A is progressing continuously, and some studies have shown its potential in immune regulation, anti-inflammatory effect and so on.

Can IL36A be used in animal experiments? 02/13/2019

Yes, IL36A can be used in animal studies to investigate its mechanism of action and potential applications.

Customer Reviews (3)

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Reviews
07/20/2022

    When conducting experiments with HSP90AA1, clear and reliable interpretation of results can be obtained.

    03/12/2021

      The IL36A product has low endotoxin levels and does not interfere with experimental results.

      08/12/2020

        IL36A has important application value in the field of drug research and development, and has achieved certain research results.

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