Recombinant Mouse Kitl protein
Cat.No. : | Kitl-554M |
Product Overview : | Recombinant Mouse Kitl protein was expressed in Escherichia coli. |
- Specification
- Gene Information
- Related Products
Description : | Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane It is required for normal hematopoietic function and plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. It also promotes mast cell adhesion, migration, proliferation, and survival. |
Source : | E.coli |
Species : | Mouse |
Form : | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 10⁵ IU/mg. |
Molecular Mass : | Approximately 18.4 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids. |
Protein length : | 165 |
AA Sequence : | MKEICGNPVTDNVKDITKLVANLPN DYMITLNYVAGMDVLPSHCWLRDMV IQLSLSLTTLLDKFSNISEGLSNYS IIDKLGKIVDDLVLCMEENAPKNIK ESPKRPETRSFTPEEFFSIFNRSID AFKDFMVASDTSDCVLSSTLGPEKD SRVSVTKPFMLPPVA |
Endotoxin : | Less than 1 EU/μg of rMuSCF as determined by LAL method. |
Purity : | >97% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | Kitl |
Official Symbol : | Kitl |
Synonyms : | KITL; kit ligand; cloud gray; C-kit ligand; Steel factor; grizzle-belly; stem cell factor; mast cell growth factor; hematopoietic growth factor KL; Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; Kitlg; contrasted; |
Gene ID : | 17311 |
mRNA Refseq : | NM_013598 |
Protein Refseq : | NP_038626 |
UniProt ID : | P20826 |
Products Types
◆ Recombinant Protein | ||
KITLG-199H | Active Recombinant Human KITLG Protein | +Inquiry |
KITLG-38C | Recombinant Chicken KITLG Protein | +Inquiry |
KITLG-2366H | Recombinant Human KITLG Protein, His-tagged | +Inquiry |
Kitl-198M | Active Recombinant Mouse Kitl Protein | +Inquiry |
Kitl-552M | Recombinant Mouse Kitl Protein | +Inquiry |
◆ Lysates | ||
KITLG-571HCL | Recombinant Human KITLG cell lysate | +Inquiry |
KITLG-583MCL | Recombinant Mouse KITLG cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionOverexpression or mutations in KITLG and its receptor have been implicated in various cancers, including gastrointestinal stromal tumors (GISTs), mastocytosis, and certain leukemias.
Given its role in cell proliferation and differentiation, KITLG has been explored as a therapeutic agent in stem cell transplantation settings, and its pathway is a target in certain cancer treatments.
Dysregulation or mutations in KITLG have been associated with certain disorders like piebaldism, a genetic condition characterized by unpigmented patches of skin and hair.
Upon binding to c-KIT, KITLG activates the SCF signaling pathways, which include the MAPK, PI3K, and JAK/STAT pathways, influencing cell survival, proliferation, and differentiation.
KITLG binds to the c-KIT receptor, a tyrosine kinase receptor, leading to its dimerization and autophosphorylation, which activates downstream signaling pathways.
Given its role in cell proliferation and differentiation, KITLG has been explored as a therapeutic agent in stem cell transplantation settings, and its pathway is a target in certain cancer treatments.
KITLG and its receptor c-KIT are involved in melanogenesis. Their interaction regulates the proliferation and migration of melanocytes, influencing skin, hair, and eye pigmentation.
Customer Reviews (3)
Write a reviewReliable for immunoprecipitation assay.
No contamination, very clean.
Worked perfectly in Western.
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