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Recombinant Mouse Kitl protein

Cat.No. : Kitl-554M
Product Overview : Recombinant Mouse Kitl protein was expressed in Escherichia coli.
  • Specification
  • Gene Information
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Description : Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane It is required for normal hematopoietic function and plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. It also promotes mast cell adhesion, migration, proliferation, and survival.
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 10⁵ IU/mg.
Molecular Mass : Approximately 18.4 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids.
Protein length : 165
AA Sequence : MKEICGNPVTDNVKDITKLVANLPN DYMITLNYVAGMDVLPSHCWLRDMV IQLSLSLTTLLDKFSNISEGLSNYS IIDKLGKIVDDLVLCMEENAPKNIK ESPKRPETRSFTPEEFFSIFNRSID AFKDFMVASDTSDCVLSSTLGPEKD SRVSVTKPFMLPPVA
Endotoxin : Less than 1 EU/μg of rMuSCF as determined by LAL method.
Purity : >97% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : Kitl
Official Symbol : Kitl
Synonyms : KITL; kit ligand; cloud gray; C-kit ligand; Steel factor; grizzle-belly; stem cell factor; mast cell growth factor; hematopoietic growth factor KL; Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; Kitlg; contrasted;
Gene ID : 17311
mRNA Refseq : NM_013598
Protein Refseq : NP_038626
UniProt ID : P20826

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does KITLG influence stem cell factor (SCF) signaling pathways? 11/10/2022

Overexpression or mutations in KITLG and its receptor have been implicated in various cancers, including gastrointestinal stromal tumors (GISTs), mastocytosis, and certain leukemias.

What are the implications of KITLG in oncogenesis and tumor progression? 07/20/2022

Given its role in cell proliferation and differentiation, KITLG has been explored as a therapeutic agent in stem cell transplantation settings, and its pathway is a target in certain cancer treatments.

What role does KITLG play in melanogenesis and pigmentation? 01/17/2022

Dysregulation or mutations in KITLG have been associated with certain disorders like piebaldism, a genetic condition characterized by unpigmented patches of skin and hair.

Are there any known disorders associated with mutations or dysregulation of KITLG? 06/22/2020

Upon binding to c-KIT, KITLG activates the SCF signaling pathways, which include the MAPK, PI3K, and JAK/STAT pathways, influencing cell survival, proliferation, and differentiation.

What is the primary biological function of KITLG in the hematopoietic system? 04/12/2020

KITLG binds to the c-KIT receptor, a tyrosine kinase receptor, leading to its dimerization and autophosphorylation, which activates downstream signaling pathways.

How has KITLG been explored as a therapeutic target or agent in clinical settings? 02/10/2020

Given its role in cell proliferation and differentiation, KITLG has been explored as a therapeutic agent in stem cell transplantation settings, and its pathway is a target in certain cancer treatments.

How does KITLG interact with its receptor, c-KIT? 12/21/2019

KITLG and its receptor c-KIT are involved in melanogenesis. Their interaction regulates the proliferation and migration of melanocytes, influencing skin, hair, and eye pigmentation.

Customer Reviews (3)

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Reviews
01/23/2022

    Reliable for immunoprecipitation assay.

    03/01/2021

      No contamination, very clean.

      02/09/2020

        Worked perfectly in Western.

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