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Recombinant Mouse Cntf protein

Cat.No. : Cntf-565M
Product Overview : Recombinant Mouse Cntf protein was expressed in Escherichia coli.
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Description : Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Furthermore, the protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition, CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress. Recombinant murine CNTF containing 198 amino acids and it shares 82 % and 95 % a.a. sequence identity with human and rat CNTF.
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered concentrated solution in 2 × PBS, pH 7.4, 2 % trehalose.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 10⁴ IU/mg.
Molecular Mass : Approximately 22.5 kDa, a single non-glycosylated polypeptide chain containing 197 amino acids.
Protein length : 197
AA Sequence : AFAEQSPLTLHRRDLCSRSIWLARK IRSDLTALMESYVKHQGLNKNISLD SVDGVPVASTDRWSEMTEAERLQEN LQAYRTFQGMLTKLLEDQRVHFTPT EGDFHQAIHTLTLQVSAFAYQLEEL MALLEQKVPEKEADGMPVTIGDGGL FEKKLWGLKVLQELSQWTVRSIHDL RVISSHHMGISAHESHYGAKQM
Endotoxin : Less than 1 EU/µg of rMuCNTF as determined by LAL method.
Purity : >95% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : Cntf
Official Symbol : Cntf
Synonyms : CNTF; ciliary neurotrophic factor; AI429687; MGC41235;
Gene ID : 12803
mRNA Refseq : NM_170786
Protein Refseq : NP_740756
UniProt ID : P51642

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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How does CNTF affect glial cell function? 08/18/2021

CNTF plays a crucial role in regulating glial cell function. It can modulate astrocyte and oligodendrocyte differentiation, promote myelination, and regulate the release of neurotrophic factors from glial cells. CNTF also influences glial cell-mediated immune responses and their interaction with neurons in various pathological conditions.

What are the signaling pathways activated by CNTF in neurons and glial cells? 11/28/2020

CNTF activates multiple signaling pathways, including JAK/STAT, PI3K/AKT, and MAPK/ERK, in both neurons and glial cells. These pathways play crucial roles in regulating gene expression, cell survival, differentiation, and synaptic plasticity.

What is the role of CNTF in neuroinflammation? 09/08/2019

CNTF plays a significant role in modulating neuroinflammatory responses. It can regulate the activation and function of microglia and astrocytes, leading to the production of pro-inflammatory or anti-inflammatory factors. CNTF also influences the recruitment and activation of immune cells in the central nervous system.

How does CNTF affect synaptic plasticity? 10/22/2016

CNTF has been shown to modulate synaptic plasticity, which underlies learning and memory processes. It promotes the formation and stabilization of synapses, regulates neurotransmitter release, and influences synaptic strength and plasticity through its interaction with specific receptors and downstream signaling molecules.

What are the challenges in developing CNTF-based therapies? 10/16/2016

Developing CNTF-based therapies faces challenges such as the need for targeted delivery to specific brain regions, potential side effects due to systemic administration, and the need to optimize dosing and treatment regimens. Additionally, understanding the precise mechanisms of CNTF action and identifying suitable patient populations for treatment are important considerations.

How does CNTF promote neuronal survival and regeneration? 04/14/2016

CNTF exerts neuroprotective effects by promoting neuronal survival and regeneration through the activation of various intracellular signaling pathways. It enhances the expression of neurotrophic factors, regulates apoptosis-related genes, and stimulates axonal outgrowth and synaptic remodeling.

Can CNTF be a potential therapeutic approach for neurodegenerative diseases? 02/07/2016

CNTF shows promise as a potential therapeutic approach for neurodegenerative diseases, such as amyotrophic lateral sclerosis and retinal degeneration. Its neuroprotective and neurotrophic effects make it a potential candidate for promoting neuronal survival, preventing neurodegeneration, and improving functional recovery in these conditions.

Customer Reviews (3)

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03/04/2020

    It is highly durable and meets the requirements for long-term experiments.

    06/02/2017

      A trusted aide, this reagent is instrumental on the path of scientific inquiry.

      09/01/2016

        Employing this protein reagent allows me to obtain precise structural information in protein interaction studies, offering critical insights for a deeper understanding of protein function.

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