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Recombinant Mouse Cntf

Cat.No.: Cntf-565M
Product Overview: Recombinant Mouse Cntf produced in E. coli is approximately 22.4 kDa, a single non-glycosylated polypeptide chain containing 197 amino acids.
Description: Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Furthermore, the protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition, CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress. Recombinant murine CNTF containing 198 amino acids and it shares 82% and 95% a.a. sequence identity with human and rat CNTF.
Source: E. coli
Species: Mouse
Form: Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 2% trehalose.
Bio-activity: Fully biologically active when compared to standard. The ED 50 determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of >3.3 × 10^4 IU/mg.
Molecular Mass: 22.4 kDa
AA Sequence: AFAEQSPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNISLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LTLQVSAFAY QLEELMALLE QKVPEKEADG MPVTIGDGGLFEKKLWGLKV LQELSQWTVR SIHDLRVISS HHMGISAHES HYGAKQM
Endotoxin: Less than 1 EU/μg of rMuCNTF as determined by LAL method.
Purity: >95% by SDS-PAGE and HPLC analyses.
Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name: Cntf ciliary neurotrophic factor [ Mus musculus ]
Official Symbol: Cntf
Synonyms: CNTF; ciliary neurotrophic factor; AI429687; MGC41235;
Gene ID: 12803
mRNA Refseq: NM_170786
Protein Refseq: NP_740756
Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;
Function: ciliary neurotrophic factor receptor binding; cytokine activity; growth factor activity; interleukin-6 receptor binding;

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