Recombinant Rat Il2 protein
Cat.No. : | Il2-575R |
Product Overview : | Recombinant Rat Il2 protein was expressed in Escherichia coli. |
- Specification
- Gene Information
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Description : | IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues and it shares about 82 %, 66 %, 66 %, 60 % and 54 % amino acid sequence identity with mouse, human, rhesus macaque, pig and equus caballus IL-2, respectively. |
Source : | E.coli |
Species : | Rat |
Form : | 0.2μm Filtered concentrated sterile solution in 10 mM sodium citrate, pH 4.0. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁶ IU/mg. |
Molecular Mass : | Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 135 amino acids. |
Protein length : | 135 |
AA Sequence : | APTSSPAKETQQHLEQLLLDLQVLL RGIDNYKNLKLPMMLTFKFYLPKQA TELKHLQCLENELGALQRVLDLTQS KSFHLEDAGNFISNIRVTVVKLKGS ENKFECQFDDEPATVVEFLRRWIAI CQSIISTMTQ |
Endotoxin : | Less than 1 EU/μg of rRtIL-2 as determined by LAL method. |
Purity : | >97% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | Il2 |
Official Symbol : | Il2 |
Synonyms : | T-cell Growth Factor, CGF |
Gene ID : | 116562 |
mRNA Refseq : | NM_053836 |
Protein Refseq : | NP_446288 |
UniProt ID : | P17108 |
Products Types
◆ Recombinant Protein | ||
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Il2-57M | Active Recombinant Mouse Il2 Protein (Ala21-Gln169), C-His tagged, Animal-free, Carrier-free | +Inquiry |
IL2-158P | Active Recombinant Porcine IL2 Protein | +Inquiry |
IL2-41H | Recombinant Human IL2 Protein | +Inquiry |
IL2-14H | Recombinant Human IL2 Protein | +Inquiry |
◆ Lysates | ||
IL2-5234HCL | Recombinant Human IL2 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionIL2 promotes the formation of memory T-cells.
IL2 has a pro-tumorigenic role in the tumor microenvironment.
IL2 synergizes with IL-12 in a mixed cytokine environment.
IL2 upregulates the expression of PD-1 checkpoint inhibitors.
IL2 is rapidly internalized by activated T-cells.
IL2 induces M1 macrophage polarization.
IL2 increases blood-brain barrier permeability.
IL2's affinity for its receptor decreases under hypoxic conditions.
IL2's bioactivity is enhanced by glycosylation.
IL2 promotes the differentiation of Th17 cells.
Customer Reviews (3)
Write a reviewPotent in T-cell activation.
Suitable for cytokine assays.
High stability.
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