Recombinant Mouse Pf4 protein
Cat.No. : | Pf4-611M |
Product Overview : | Recombinant Mouse Pf4 protein was expressed in Escherichia coli. |
- Specification
- Gene Information
- Related Products
Description : | CXCL4 (PF4) is a small cytokine belonging to the CXC chemokine family and it is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. CXCL4 interacts with a splice variant of the chemokine receptor CXCR3. Recombinant mouse CXCL4 contains 76 amino acids which is a single non-glycosylated polypeptide chain. Specifically, Human and mouse CXCL4 share about a 60 % identity. |
Source : | E.coli |
Species : | Mouse |
Form : | Lyophilized from a 0.2μm filtered concentrated solution in 20 mM PB, 1.5 M NaCl, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml. |
Molecular Mass : | Approximately 8.2 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids. |
Protein length : | 76 |
AA Sequence : | VTSAGPEESDGDLSCVCVKTISSGI HLKHITSLEVIKAGRHCAVPQLIAT LKNGRKICLDRQAPLYKKVIKKILE S |
Endotoxin : | Less than 1 EU/μg of rMuPF-4/CXCL4 as determined by LAL method. |
Purity : | >97% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | Pf4 |
Official Symbol : | Pf4 |
Synonyms : | PF4; platelet factor 4; PF-4; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; Cxcl4; Scyb4; |
Gene ID : | 56744 |
mRNA Refseq : | NM_019932 |
Protein Refseq : | NP_064316 |
UniProt ID : | Q9Z126 |
Products Types
◆ Recombinant Protein | ||
PF4-1650H | Recombinant Human PF4 Protein, His (Fc)-Avi-tagged | +Inquiry |
PF4-245H | Active Recombinant Human PF4 Protein (Glu32-Ser101), N-His tagged, Animal-free, Carrier-free | +Inquiry |
Pf4-4803M | Active Recombinant Mouse Pf4 Protein | +Inquiry |
Pf4-4804M | Recombinant Mouse Pf4 Protein, Myc/DDK-tagged | +Inquiry |
PF4-166P | Active Recombinant Human PF4 Protein | +Inquiry |
◆ Native Protein | ||
PF4-253H | Native Human Platelet Factor 4 | +Inquiry |
◆ Lysates | ||
PF4-3283HCL | Recombinant Human PF4 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionSome research has explored the development of antibodies targeting PF4 to mitigate its pro-inflammatory effects, although such treatments are still in the early stages of investigation.
PF4 can promote inflammation by recruiting and activating immune cells, such as monocytes and neutrophils, at the site of inflammation.
Management often involves discontinuing heparin and initiating alternative anticoagulation strategies, such as direct thrombin inhibitors or fondaparinux.
Yes, elevated PF4 levels have been associated with various inflammatory states, making it a potential biomarker for certain diseases.
ELISA (enzyme-linked immunosorbent assay) is commonly used to detect anti-PF4/heparin antibodies in patients suspected of having HIT.
Customer Reviews (3)
Write a reviewthe PF4 protein's well-documented properties and thoroughly tested functionality give me the confidence to integrate it seamlessly into my experimental setup.
Its impeccable purity and reliability ensure accurate and reproducible results, providing a solid foundation for my research.
he PF4 protein is an exceptional choice that meets the highest standards of quality, perfectly catering to my experimental requirements.
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