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Recombinant Mouse Pf4 protein

Cat.No. : Pf4-611M
Product Overview : Recombinant Mouse Pf4 protein was expressed in Escherichia coli.
  • Specification
  • Gene Information
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Description : CXCL4 (PF4) is a small cytokine belonging to the CXC chemokine family and it is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. CXCL4 interacts with a splice variant of the chemokine receptor CXCR3. Recombinant mouse CXCL4 contains 76 amino acids which is a single non-glycosylated polypeptide chain. Specifically, Human and mouse CXCL4 share about a 60 % identity.
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered concentrated solution in 20 mM PB, 1.5 M NaCl, pH 7.4.
Bio-activity : Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml.
Molecular Mass : Approximately 8.2 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.
Protein length : 76
AA Sequence : VTSAGPEESDGDLSCVCVKTISSGI HLKHITSLEVIKAGRHCAVPQLIAT LKNGRKICLDRQAPLYKKVIKKILE S
Endotoxin : Less than 1 EU/μg of rMuPF-4/CXCL4 as determined by LAL method.
Purity : >97% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : Pf4
Official Symbol : Pf4
Synonyms : PF4; platelet factor 4; PF-4; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; Cxcl4; Scyb4;
Gene ID : 56744
mRNA Refseq : NM_019932
Protein Refseq : NP_064316
UniProt ID : Q9Z126

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Are there any therapeutic approaches targeting PF4 protein? 02/17/2023

Some research has explored the development of antibodies targeting PF4 to mitigate its pro-inflammatory effects, although such treatments are still in the early stages of investigation.

How does PF4 protein contribute to the pathogenesis of inflammation? 11/11/2022

PF4 can promote inflammation by recruiting and activating immune cells, such as monocytes and neutrophils, at the site of inflammation.

What are the potential treatment options for patients with HIT? 04/24/2017

Management often involves discontinuing heparin and initiating alternative anticoagulation strategies, such as direct thrombin inhibitors or fondaparinux.

Can PF4 protein be used as a biomarker for other conditions? 06/20/2016

Yes, elevated PF4 levels have been associated with various inflammatory states, making it a potential biomarker for certain diseases.

What diagnostic methods are used to assess PF4 protein levels? 01/30/2016

ELISA (enzyme-linked immunosorbent assay) is commonly used to detect anti-PF4/heparin antibodies in patients suspected of having HIT.

Customer Reviews (3)

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Reviews
09/07/2022

    the PF4 protein's well-documented properties and thoroughly tested functionality give me the confidence to integrate it seamlessly into my experimental setup.

    06/30/2018

      Its impeccable purity and reliability ensure accurate and reproducible results, providing a solid foundation for my research.

      12/12/2017

        he PF4 protein is an exceptional choice that meets the highest standards of quality, perfectly catering to my experimental requirements.

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