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Recombinant Human ME2

Cat.No. : ME2-644H
Product Overview : Recombinant Human ME2 produced in E. coli is approximately 63.5 kDa, a single non-glycosylated polypeptide chain containing 567 amino acids.
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  • Gene Information
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Description : NAD-dependent malic enzyme (ME2), mitochondrial is a protein that in humans is encoded by the ME2 gene. This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, which catalyzes the oxidative decarboxylation of malate to pyruvate. Three different isoforms of ME are known to be in mammalian tissues: a strictly cytosolic NADP+-dependent enzyme, an NADP+-dependent mitochondriail isoform, and a mitochondrial isoenzyme that is able to use both NAD+ and NADP+ but is more effective with NAD+. The mammalian isoforms size is about 62-64 kDa. A native size of 240,000 Da proposes a tetrameric structure for the active enzyme.
Source : E. coli
Species : Human
Form : Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Molecular Mass : 63.5 kDa
AA Sequence : MLHIKEKGKP LMLNPRTNKG MAFTLQERQM LGLQGLLPPK IETQDIQALR FHRNLKKMTS PLEKYIYIMG IQERNEKLFY RILQDDIESL MPIVYTPTVG LACSQYGHIF RRPKGLFISI SDRGHVRSIV DNWPENHVKA VVVTDGERIL GLGDLGVYGM GIPVGKLCLY TACAGIRPDR CLPVCIDVGT DNIALLKDPF YMGLYQKRDR TQQYDDLIDE FMKAITDRYG
Endotoxin : Less than 1 EU/μg of rHuME2 as determined by LAL method.
Purity : >95% by SDS-PAGE and HPLC analyses.
Usage : This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage : This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution : We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : ME2 malic enzyme 2, NAD(+)-dependent, mitochondrial [ Homo sapiens ]
Official Symbol : ME2
Synonyms : ME2; malic enzyme 2, NAD(+)-dependent, mitochondrial; NAD-dependent malic enzyme, mitochondrial; NAD-ME; malate dehydrogenase; pyruvic-malic carboxylase; ODS1;
Gene ID : 4200
mRNA Refseq : NM_001168335
Protein Refseq : NP_001161807
MIM : 154270
UniProt ID : P23368
Chromosome Location : 18q21
Pathway : Pyruvate metabolism, organism-specific biosystem; Pyruvate metabolism, conserved biosystem; gluconeogenesis I, organism-specific biosystem;
Function : NAD binding; electron carrier activity; malate dehydrogenase (decarboxylating) activity; malate dehydrogenase (oxaloacetate-decarboxylating) activity; metal ion binding; oxidoreductase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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