"MIF" Related Products

Expand
Expand

Recombinant Human MIF, His-tagged

Cat.No.: MIF-650H
Product Overview: Recombinant Human MIF produced in E. coli is approximately 13.5 kDa, a single non-glycosylated polypeptide chain containing 117 amino acids, with 6×His at C-terminus.
Description: Human MIF consists of two α-helices and six β-strands, four of which form a β-sheet. The two remaining β-strands interact with other MIF molecules, creating a trimer. Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position 1. Amino acids (a.a.) 50 - 65 have also been suggested to contain thiol-protein oxidoreductase activity. MIF has proinflammatory cytokine activity centered around a.a. 49-65. On fibroblasts, MIF induces,IL-1,IL-8and MMP expression; on macrophages, MIF stimulates NO production and TNF-α release folllowing IFN-γ activation. MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction. Human MIF is active on mouse cells. Human MIF is 90 %, 94 %, 9 5%, and 90 % aa identical to mouse, bovine, porcine and rat MIF, respectively.
Source: E. coli
Species: Human
Tag: His
Form: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity: Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.
Molecular Mass: 13.5 kDa
AA Sequence: MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFALEHHH HHH
Endotoxin: Less than 1 EU/μg of rHuMIF, His as determined by LAL method.
Purity: >95% by SDS-PAGE and HPLC analyses.
Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name: MIF macrophage migration inhibitory factor (glycosylation-inhibiting factor) [ Homo sapiens ]
Official Symbol: MIF
Synonyms: MIF; macrophage migration inhibitory factor (glycosylation-inhibiting factor); GLIF; macrophage migration inhibitory factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase; MMIF;
Gene ID: 4282
mRNA Refseq: NM_002415
Protein Refseq: NP_002406
MIM: 153620
UniProt ID: P14174
Chromosome Location: 22q11.23
Pathway: Adipogenesis, organism-specific biosystem; Phenylalanine metabolism, organism-specific biosystem; Phenylalanine metabolism, conserved biosystem; Tyrosine metabolism, organism-specific biosystem; Tyrosine metabolism, conserved biosystem;
Function: cell surface binding; chemoattractant activity; cytokine activity; cytokine receptor binding; dopachrome isomerase activity; isomerase activity; phenylpyruvate tautomerase activity; protein binding; receptor binding;

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.