Recombinant Human HRAS(Mature form), His-tagged HRAS-229H

Cat.No.:	HRAS-229H
Product Overview:	Recombinant human RAS1 (2-186) was expressed in E.coli cells using an N-terminal His tag. The RAS1 mature form shows a higher activity compared to RAS1 (1-189) precursor form.
Description:	The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). RAS and several other cellular proteins with similar biochemical properties are collectively known as G-proteins and they play key roles in a wide variety of cellular activities, including cell growth, differentiation, secretion, and protein trafficking. There are three forms of RAS gene in cells termed H-RAS, N-RAS, and K-RAS. RAS proteins play a direct causal role in human cancer and in other diseases. Mutant H-RAS, N-RAS, and K-RAS occur in varying frequencies in different tumor types. Other members of the RAS superfamily may also contribute to cancer.
Source:	E. coli
Species:	Human
Tag:	His
Form:	Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Molecular Mass:	~23 kDa
Purity:	>95% by densitometry
Applications:	Western Blot
Storage:	Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration:	0.1 μg/μl

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Gene Name:	HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog [ Homo sapiens ]
Official Symbol:	HRAS
Synonyms:	HRAS; v-Ha-ras Harvey rat sarcoma viral oncogene homolog; HRAS1; GTPase HRas; p21ras; H-Ras-1; p19 H-RasIDX protein; c-has/bas p21 protein; transforming protein p21; Ha-Ras1 proto-oncoprotein; c-ras-Ki-2 activated oncogene; GTP- and GDP-binding peptide B; transformation gene: oncogene HAMSV; Ras family small GTP binding protein H-Ras; CTLO; HAMSV; K-RAS; N-RAS; RASH1; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1;
Gene ID:	3265
mRNA Refseq:	NM_001130442
Protein Refseq:	NP_001123914
MIM:	190020
UniProt ID:	P01112
Chromosome Location:	11p15.5
Pathway:	ARMS-mediated activation, organism-specific biosystem; Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Activation of RAS in B Cells, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Axon guidance, organism-specific biosystem;
Function:	GTP binding; GTPase activity; nucleotide binding; protein C-terminus binding; protein binding;