Cat.No.: |
HRAS-229H |
Product Overview: |
Recombinant human RAS1 (2-186) was expressed in E.coli cells using an N-terminal His tag. The RAS1 mature form shows a higher activity compared to RAS1 (1-189) precursor form. |
Description: |
The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). RAS and several other cellular proteins with similar biochemical properties are collectively known as G-proteins and they play key roles in a wide variety of cellular activities, including cell growth, differentiation, secretion, and protein trafficking. There are three forms of RAS gene in cells termed H-RAS, N-RAS, and K-RAS. RAS proteins play a direct causal role in human cancer and in other diseases. Mutant H-RAS, N-RAS, and K-RAS occur in varying frequencies in different tumor types. Other members of the RAS superfamily may also contribute to cancer. |
Source: |
E. coli |
Species: |
Human |
Tag: |
His |
Form: |
Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. |
Molecular Mass: |
~23 kDa |
Purity: |
>95% by densitometry |
Applications: |
Western Blot |
Storage: |
Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Concentration: |
0.1 μg/μl |