Active Recombinant Human PCSK1, His-tagged

• Cat.No.: PCSK1-87H
• Product Overview: Recombinant Human PCSK1 (Ser111-Arg617) fused with 10­His tag at C­terminal, was expressed in Mouse NSO Cells.

Specification

• Description: The protein encoded by this gene belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a type I proinsulin-processing enzyme that plays a key role in regulating insulin biosynthesis. It is also known to cleave proopiomelanocortin, prorenin, proenkephalin, prodynorphin, prosomatostatin and progastrin. Mutations in this gene are thought to cause obesity. This encoded protein is associated with carcinoid tumors. Mutations in this gene have been associated with susceptibility to obesity and proprotein convertase 1/3 deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ser111
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, acetyl-Arg-Glu-Lys-Arg-7-amino-trifluoromethyl coumarin (Ac-REKR-AFC). The specific activity is >8 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 57 kDa; SDS-PAGE:66 kDa, reducing conditions.
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: PCSK1 proprotein convertase subtilisin/kexin type 1 [ Homo sapiens(human) ]
• Official Symbol: PCSK1
• Synonyms: PCSK1; PC1; PC3; NEC1; SPC3; BMIQ12; proprotein convertase subtilisin/kexin type 1; neuroendocrine convertase 1; NEC 1; prohormone convertase 1; prohormone convertase 3; EC 3.4.21.93
• Gene ID: 5122
• mRNA Refseq: NM_000439
• Protein Refseq: NP_000430
• MIM: 162150
• UniProt ID: P29120
• Chromosome Location: 5q15-q21
• Pathway: Incretin Synthesis; Insulin Processing; Peptide hormone biosynthesis
• Function: serine-type endopeptidase activity