Recombinant Rat Tnf

Cat. No.: Tnf-71R
Product Overview: Recombinant Rat Tumor Necrosis Factor produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17339.44 Dalton. The TNF-alpha is purified by standard chromatographic techniques.
Description: Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF"s primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
Source: Escherichia Coli.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Ser-Ser.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAG.
Formulation: The protein was lyophilized with no additives.
Solubility: It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05ng/ml, corresponding to a Specific Activity of 5x107 IU/mg.
Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.25 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of TNF-as a Reference Standard.
Storage: Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name: Tnf tumor necrosis factor (TNF superfamily, member 2) [ Rattus norvegicus ]
Synonyms: Tnf, tumor necrosis factor (TNF superfamily, member 2); Tnfa; RATTNF; MGC124630; TNF-alpha; Tnf; 20p12; tumor necrosis factor superfamily member 2; Tumor necrosis factor, alpha (cachetin); MGC124630; Tumor necrosis factor alpha (cachetin); tumor necrosis factor superfamily, member 2
Gene ID: 24835
mRNA Refseq: NM_012675
Protein Refseq: NP_036807
UniProt ID: P16599
Chromosome Location: 20p12
Pathway: Adipocytokine signaling pathway; Allograft rejection; Alzheimer"s disease; Amyotrophic lateral sclerosis (ALS); Apoptosis; Asthma; Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Graft-versus-host disease; Hematopoietic cell lineage; Hypertrophic cardiomyopathy (HCM); MAPK signaling pathway; Natural killer cell mediated cytotoxicity; RIG-I-like receptor signaling pathway; emic lupus erythematosus; T cell receptor signaling pathway; TGF-beta signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus; Type II diabetes mellitus
Function: cytokine activity; identical protein binding; tumor necrosis factor receptor binding; protein binding

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