Active Recombinant Human PARP1, MYC-tagged
Cat.No. : | PARP1-444H |
Product Overview : | Human PARP-1 [ARTD1] (aa 656-1014) is fused to a MYC and His-tag. |
- Specification
- Gene Information
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Description : | PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-γ to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. |
Source : | Baculovirus infected Sf21 Cells |
Species : | Human |
Tag : | MYC |
Form : | Liquid. In 50mM TRIS-HCl, pH 7.5, containing 300mM sodium chloride, 250mM imidazole, 2mM β-mercaptoethanol, 0.2% NP-40 and 10% glycerol. |
Bio-activity : | ~50ng are required for poly(ADP-ribose) formation. |
Purity : | ≥90% (SDS-PAGE) |
Stability : | Stable for at least 6 months after receipt when stored at -80°C. |
Storage : | Short Term Storage: -20°C; Long Term Storage: -80°C. After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles. |
Concentration : | Lot dependent (0.2-1.5mg/ml) |
Gene Name : | PARP1 poly (ADP-ribose) polymerase 1 [ Homo sapiens ] |
Official Symbol : | PARP1 |
Synonyms : | PARP1; poly (ADP-ribose) polymerase 1; ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) , ADPRT, poly (ADP ribose) polymerase family, member 1 , PPOL; poly [ADP-ribose] polymerase 1; PARP; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly[ADP-ribose] synthase 1; poly(ADP-ribosyl)transferase; ADP-ribosyltransferase NAD(+); NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); PPOL; ADPRT; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; |
Gene ID : | 142 |
mRNA Refseq : | NM_001618 |
Protein Refseq : | NP_001609 |
MIM : | 173870 |
UniProt ID : | P09874 |
Chromosome Location : | 1q41-q42 |
Pathway : | BER complex, organism-specific biosystem; BER complex, conserved biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Caspase cascade in apoptosis, organism-specific biosystem; FAS pathway and Stress induction of HSP regulation, organism-specific biosystem; Notch-mediated HES/HEY network, organism-specific biosystem; |
Function : | DNA binding; NAD binding; NAD+ ADP-ribosyltransferase activity; metal ion binding; protein N-terminus binding; protein binding; transcription factor binding; transferase activity, transferring glycosyl groups; zinc ion binding; |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionYes, overactivation of the PARP1 protein can lead to apoptosis or necrosis of cells, which may be exploited in the treatment of certain diseases.
The PARP1 protein can repair DNA damage by attaching ADP-ribose groups to damaged DNA strands and recruiting other repair proteins.
In addition to PARP1's involvement in direct DNA repair, it can also modulate the selection of repair pathways and initiate other DNA damage sensing mechanisms.
Some studies have proposed that the interaction of PARP1 protein with DNA damage in tumor immunotherapy may affect the efficacy of immunotherapy.
Yes, the PARP1 protein is involved in the normal regulation of the cell cycle, especially G1/S and G2/M switching during DNA damage.
Overactivation of PARP1 depletes energy stores such as NAD+ and ATP and leads to apoptosis, or cell death.
Customer Reviews (3)
Write a reviewThis range of applications of PARP1 is breathtaking, and it can play an important role in any field.
This short half-life and high clearance of PARP1 make it have greater potential in the treatment of tumors, infections and other diseases, and it is expected to become a new generation of therapeutic drugs.
The purity and bioactivity of PARP1 make it a valuable resource for scientific research.
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