Active Recombinant Human PARP1, HA-tagged

• Cat.No.: PARP1-451H
• Product Overview: Human full-length inactive mutant E998K of PARP-1 is fused to a HA-tag and a His-tag.

Specification

• Description: PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. PARP-1 (E998K mutant) is an inactive form of PARP-1 which can be used as a control compound.
• Source: Baculovirus infected Sf21 Cells
• Species: Human
• Tag: HA
• Form: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 50mM imidazole, 0.2% NP-40 and 10% glycerol.
• Bio-activity: 0.5% of wild type PARP-1.
• Purity: ≥95% (SDS-PAGE)
• Applications: Control
• Stability: Stable for at least 6 months after receipt when stored at -80°C.
• Storage: Short Term Storage: -20°C; Long Term Storage: -80°C. After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
• Concentration: Lot dependent (0.2-0.5mg/ml)

Gene Information

• Gene Name: PARP1 poly (ADP-ribose) polymerase 1 [ Homo sapiens ]
• Official Symbol: PARP1
• Synonyms: PARP1; poly (ADP-ribose) polymerase 1; ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) , ADPRT, poly (ADP ribose) polymerase family, member 1 , PPOL; poly [ADP-ribose] polymerase 1; PARP; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly[ADP-ribose] synthase 1; poly(ADP-ribosyl)transferase; ADP-ribosyltransferase NAD(+); NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); PPOL; ADPRT; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
• Gene ID: 142
• mRNA Refseq: NM_001618
• Protein Refseq: NP_001609
• MIM: 173870
• UniProt ID: P09874
• Chromosome Location: 1q41-q42
• Pathway: BER complex, organism-specific biosystem; BER complex, conserved biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Caspase cascade in apoptosis, organism-specific biosystem; FAS pathway and Stress induction of HSP regulation, organism-specific biosystem; Notch-mediated HES/HEY network, organism-specific biosystem
• Function: DNA binding; NAD binding; NAD+ ADP-ribosyltransferase activity; metal ion binding; protein N-terminus binding; protein binding; transcription factor binding; transferase activity, transferring glycosyl groups; zinc ion binding