Active Recombinant Human KAT5, His-tagged

• Cat.No.: KAT5-518H
• Product Overview: Human TIP60 is fused to a His-tag.

Specification

• Description: TIP60 is the catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex is required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. TIP60 directly acetylates and activates ataxia-telangiectasia mutated (ATM). In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation.
• Source: Baculovirus infected Sf21 Cells
• Species: Human
• Tag: His
• Form: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.
• Bio-activity: 100-200ng are required for standard HAT assay.
• Purity: ≥95% (SDS-PAGE)
• Stability: Stable for at least 6 months after receipt when stored at -80°C.
• Storage: Short Term Storage: -20°C; Long Term Storage: -80°C. After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
• Concentration: Lot dependent (0.2-0.5mg/ml)

Gene Information

• Gene Name: KAT5 K(lysine) acetyltransferase 5 [ Homo sapiens ]
• Official Symbol: KAT5
• Synonyms: KAT5; K(lysine) acetyltransferase 5; HIV 1 Tat interactive protein, 60kDa , HTATIP; histone acetyltransferase KAT5; cPLA2; ESA1; HTATIP1; K acetyltransferase 5; PLIP; Tat interacting protein; 60kDa; TIP60; K-acetyltransferase 5; cPLA2 interacting protein; lysine acetyltransferase 5; cPLA(2)-interacting protein; 60 kDa Tat-interactive protein; Tat interacting protein, 60kDa; histone acetyltransferase HTATIP; HIV-1 Tat interactive protein, 60kDa; TIP; HTATIP
• Gene ID: 10524
• mRNA Refseq: NM_001206833
• Protein Refseq: NP_001193762
• MIM: 601409
• UniProt ID: Q92993
• Chromosome Location: 11q13
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; C-MYC pathway, organism-specific biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; IL-9 Signaling Pathway, organism-specific biosystem; Regulation of Androgen receptor activity, organism-specific biosystem
• Function: androgen receptor binding; contributes_to histone acetyltransferase activity; metal ion binding; protein binding; repressing transcription factor binding; transcription coactivator activity; transcription coactivator activity; transferase activity, transferring acyl groups other than amino-acyl groups