Recombinant Human SIRT1, His-tagged
Cat.No. : | SIRT1-132H |
Product Overview : | SIRT1, 254-495aa, Human, His tag, E.coli |
- Specification
- Gene Information
- Related Products
Description : | SIRT1 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. Alternative splicing results in multiple transcript variants. Recombinant human SIRT1 protein, fused to His-tag at N-terminus, was expressed in E.coli . |
Source : | E.coli |
Species : | Human |
Tag : | His-tag |
Form : | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea |
Molecular Mass : | 31.6 kDa (280 aa) |
Protein length : | 254-495aa |
AA Sequence : | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMKK IIVLTGAGVS VSCGIPDFRS RDGIYARLAV DFPDLPDPQA MFDIEYFRKD PRPFFKFAKE IYPGQFQPSL CHKFIALSDK EGKLLRNYTQ NIDTLEQVAG IQRIIQCHGS FATASCLICK YKVDCEAVRG DIFNQVVPRC PRCPADEPLA IMKPEIVFFG ENLPEQFHRA MKYDKDEVDL LIVIGSSLKV RPVALIPSSI PHEVPQILIN REPLPHLHFD VELLGDCDVI INELCHRLGG |
Purity : | >90 % by SDS - PAGE |
Storage : | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Concentration : | 0.5 mg/ml (determined by Bradford assay) |
Gene Name : | SIRT1 sirtuin 1 [ Homo sapiens ] |
Official Symbol : | SIRT1 |
Synonyms : | SIRT1; sirtuin 1; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) , sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1; NAD-dependent deacetylase sirtuin-1; SIR2L1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; SIR2-like protein 1; |
Gene ID : | 23411 |
mRNA Refseq : | NM_001142498 |
Protein Refseq : | NP_001135970 |
MIM : | 604479 |
UniProt ID : | Q96EB6 |
Chromosome Location : | 10q21 |
Pathway : | Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; E2F transcription factor network, organism-specific biosystem; Energy Metabolism, organism-specific biosystem; FoxO family signaling, organism-specific biosystem; HIF-2-alpha transcription factor network, organism-specific biosystem; Regulation of Androgen receptor activity, organism-specific biosystem; |
Function : | HLH domain binding; NOT NAD+ ADP-ribosyltransferase activity; NAD+ binding; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent protein deacetylase activity; NAD-dependent protein deacetylase activity; bHLH transcription factor binding; deacetylase activity; enzyme binding; histone binding; histone deacetylase activity; identical protein binding; metal ion binding; mitogen-activated protein kinase binding; p53 binding; protein C-terminus binding; protein binding; protein deacetylase activity; transcription corepressor activity; transcription corepressor activity; zinc ion binding; |
Products Types
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SIRT1-2014H | Recombinant Human SIRT1 Protein, His (Fc)-Avi-tagged | +Inquiry |
SIRT1-30H | Recombinant Human SIRT1 Protein, GST-tagged | +Inquiry |
SIRT1-11H | Recombinant Human SIRT1 Protein, MYC/DDK-tagged | +Inquiry |
◆ Lysates | ||
SIRT1-595HCL | Recombinant Human SIRT1 lysate | +Inquiry |
◆ Assay kits | ||
Kit-2156 | SIRT1 Inhibitor/Activator Screening Kit | +Inquiry |
Kit-2024 | SIRT1 Direct Fluorescent Screening Assay Kit | +Inquiry |
Kit-2025 | SIRT1 FRET-Based Screening Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionStudies have found that SIRT1 plays an important role in tissue regeneration. Activation of SIRT1 can promote the proliferation and differentiation of stem cells, which contributes to tissue regeneration and repair.
Activation of SIRT1 promotes autophagy, which helps to remove damaged organelles and proteins and maintain cell health.
SIRT1 regulates mitochondrial function. Activation of SIRT1 can improve mitochondrial metabolism and function, contributing to energy metabolism and cell survival.
Studies have found that SIRT1 exerts carcinogenic effects in some cancers, but exerts anti-cancer effects in others. It depends on the type of cancer and environmental factors.
SIRT1 has potential therapeutic value in drug development. The development of small molecule drugs targeting SIRT1 can be used to treat a variety of diseases, including aging, diabetes, cardiovascular diseases, and neurodegenerative diseases.
SIRT1 interacts and regulates with other sirtuins. For example, SIRT1 can regulate the expression and activity of other Sirtuins, and other Sirtuins can also regulate the activity of SIRT1.
Customer Reviews (3)
Write a reviewResulting banding is clear and bright, much more than expected.
SIRT1 has high safety, environmental protection and no pollution.
The quality is so good. It saves a lot of time. Good.
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