Recombinant Human TNFSF11, His-tagged, Animal Free
Cat.No. : | TNFSF11-144H |
Product Overview : | rhuman sRANKL is a glycosylated polypeptide chain containing 175 amino acids (70 – 244 aa of O14788 TNF11_HUMAN) and a His-tag at the N-terminal end. It has a predicted molecular mass of 21.1 kDa, however as result of glycosylation, the recombinant protein could migrate as two bands with an apparent molecular mass of 21-23 kDa in SDSPAGE. Human recombinant protein expressed in Nicotiana benthamiana. Recombinant human Receptor activator of nuclear factor kappa-B ligand (sRANK-ligand) contains a 10-His-tag at the N-terminal end, is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal–derived components or impurities. Animal free product. |
- Specification
- Gene Information
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Description : | Recombinant human RANKL is a member of TNF super family, a cytokine that play a central role in bone remodelling and disorders of mineral metabolism. It was shown to be a dendritic cell survival factor, T-cell activator and osteoclast regulator because RANKL mediates the osteoclast differentiation, survival and activation. Native RANKL is a type II trans-membrane protein with an extracellular binding domain that interacts with RANK and OPG receptors. OPG protects the skeleton from excessive bone resorption by binding to RANKL and preventing it from binding to its receptor, RANK. Thus, RANKL/OPG ratio became an important determinant of bone mass and skeletal integrity. In addition, this protein was shown to activate anti-apoptotic kinase AKT/PKB through a signalling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TREAF). Recent findings shown that OPG/RANK/RANKL system has been identifies as a possible mediator of arterial calcification suggesting common links between osteoporosis and vascular diseases. |
Source : | Nicotiana benthamiana |
Species : | Human |
Tag : | His |
Form : | Recombinant human RANKL is lyophilized from 10mM Phosphate Potasium buffer pH 8 and 0.2M NaCl. |
Molecular Mass : | rhuman sRANKL is a glycosylated polypeptide chain containing 175 amino acids (70 – 244 aa of O14788 TNF11_HUMAN) and a His-tag at the N-terminal end. It has a predicted molecular mass of 21.1 kDa, however as result of glycosylation, the recombinant prot |
AA Sequence : | HHHHHHHHHHEKAMVDGSWLDLAKR SKLEAQPFAHLTINATDIPSGSHKV SLSSWYHDRGWAKISNMTFSNGKLI VNQDGFYYLYANICFRHHETSGDLA TEYLQLMVYVTKTSIKIPSSHTLMK GGSTKYWSGNSEFHFYSINVGGFFK LRSGEEISIEVSNPSLLDPDQDATY FGAFKVRDID |
Endotoxin : | < 0.04 eu ug protein (lal> |
Purity : | >97% by SDS-PAGE gel |
Applications : | Western Blot, Immunogen. |
Storage : | This lyophilized preparation is stable at 2-8o C for short term, long storage it should be kept at -20oC. Reconstituted protein should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended. |
Reconstitution : | Lyophilized protein should be reconstituted in water to a concentration of 200 ng/μl. Optimal concentration should be determined for specific application and cell lines. Optimal reconstitution please follow batch Quality Control sheet instructions. |
Gene Name : | TNFSF11 tumor necrosis factor (ligand) superfamily, member 11 [ Homo sapiens ] |
Official Symbol : | TNFSF11 |
Synonyms : | TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11; CD254; ODF; OPGL; RANKL; TRANCE; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; sOdf; OPTB2; hRANKL2; |
Gene ID : | 8600 |
mRNA Refseq : | NM_003701 |
Protein Refseq : | NP_003692 |
MIM : | 602642 |
UniProt ID : | O14788 |
Chromosome Location : | 13q14 |
Pathway : | Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; IL6-mediated signaling events, organism-specific biosystem; Osteoblast Signaling, organism-specific biosystem; Osteoclast Signaling, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem; |
Function : | cytokine activity; cytokine activity; receptor activity; tumor necrosis factor receptor binding; tumor necrosis factor receptor superfamily binding; |
Products Types
◆ Recombinant Protein | ||
Tnfsf11-1258M | Recombinant Mouse Tnfsf11 Protein, His-tagged | +Inquiry |
Tnfsf11-6551M | Active Recombinant Mouse Tnfsf11 Protein | +Inquiry |
TNFSF11-210C | Active Recombinant Cynomolgus TNFSF11 protein(Gly136-Asp317), hFc-tagged | +Inquiry |
Tnfsf11-9481M | Recombinant Mouse Tnfsf11 Protein, His (Fc)-Avi-tagged | +Inquiry |
TNFSF11-302H | Active Recombinant Human TNFSF11 Protein (Glu143-Asp317), C-His tagged, Animal-free, Carrier-free | +Inquiry |
◆ Lysates | ||
TNFSF11-001CCL | Recombinant Cynomolgus TNFSF11 cell lysate | +Inquiry |
TNFSF11-1093HCL | Recombinant Human TNFSF11 cell lysate | +Inquiry |
TNFSF11-1586MCL | Recombinant Mouse TNFSF11 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionIt is possible to detect the expression level of TNFSF11 protein to help diagnose tumors and other diseases, and to assess their prognosis and treatment effectiveness.
At present, a variety of drugs have been developed to target TNFSF11 proteins and their related signaling pathways, including inhibiting their expression and inhibiting their binding to receptors, etc., for the treatment of tumors and other bone metabolism-related diseases.
Studies have shown that TNFSF11 proteins can be involved in the regulation of infectious diseases, such as playing a role in some viral infections.
There are differences in the expression of TNFSF11 proteins in different tissues and organs, and they are expressed in tissues and organs such as the immune system, bones, and teeth, and the expression level may be affected by many factors.
Studies have shown that TNFSF11 proteins can be involved in the regulation of autoimmune diseases, such as in autoimmune diseases such as rheumatoid arthritis.
TNFSF11 protein belongs to the TNF superfamily and has certain structural and functional similarities with other members of the TNF superfamily.
Customer Reviews (3)
Write a reviewThe reproducibility of TNFSF11 is so good that the results are highly consistent regardless of the conditions under which the experiment or application is performed, greatly reducing the experimental error.
Green production process of TNFSF11 is highly commendable, which uses the most advanced biotechnology to increase production efficiency and reduce environmental impact.
The short half-life and high clearance of TNFSF11 make it better able to adapt to changing treatment needs and provide patients with more flexible treatment options.
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