Recombinant Human RSPO1, His-tagged, Biotinylated

• Cat.No.: RSPO1-265H
• Product Overview: Human Rspondin-1 Protein, Biotinylated (Biotinylated RSPO1) Ser 21 - Ala 263, was labeled with biotin based on rhRSPO1 Protein, which was produced in human 293 cells (HEK293).

Specification

• Description: This gene is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
• Molecular Mass: Biotinylated RSPO1, fused with 6×His tag at the C-terminus, has a calculated MW of 27.6 kDa. The predicted N-terminus is Ser 21 . DTT-reduced Protein migrates as 40 kDa due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the Biotinylated RSPO1 by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Gene Information

• Gene Name: RSPO1 R-spondin 1 [ Homo sapiens ]
• Official Symbol: RSPO1
• Synonyms: RSPO1; R-spondin 1; R spondin homolog (Xenopus laevis); R-spondin-1; FLJ40906; RSPONDIN; R-spondin homolog; roof plate-specific spondin-1; RSPO; CRISTIN3; RP11-566C13.1
• Gene ID: 284654
• mRNA Refseq: NM_001038633
• Protein Refseq: NP_001033722
• MIM: 609595
• UniProt ID: Q2MKA7
• Chromosome Location: 1p34.2
• Pathway: Wnt signaling network, organism-specific biosystem
• Function: heparin binding; protein binding; receptor binding