Active Recombinant Human VEGFA, Biotinylated

• Cat.No.: VEGFA-272H
• Product Overview: Recombinant Human VEGFA (Ala 27 - Arg 191) labeled with biotin, was expressed in HEK293 cells.

Specification

• Source: HEK293
• Species: Human
• Predicted N Terminal: Ala 27
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized VEGFR2/R3-Fc at 5 µg/mL (100 µl/well) can bind Biotinylated rh VEGF165 with a linear range of 0.5-5 ng/mL.Validated binding test against Human Neuropilin-1 Protein, C-Fc Tag in a functional ELISA.
• Molecular Mass: Has a calculated MW of 19 kDa (monomer). As a result of glycosylation, DTT-reduced Protein migrates as 24 kDa (monomer) and non-reduced protein migrates as 43 kDa (homodimer) in SDS-PAGE .
• Protein length: Ala 27 - Arg 191
• Endotoxin: Less than 1.0 EU per μg of the Biotinylated rh VEGF165 by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Applications: ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF; vascular permeability factor; VEGF; MVCD1; MGC70609
• Gene ID: 7422
• mRNA Refseq: NM_001171626
• Protein Refseq: NP_001165097
• MIM: 192240
• UniProt ID: P15692
• Chromosome Location: 6p12
• Pathway: Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Endochondral Ossification, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem
• Function: cell surface binding; chemoattractant activity; cytokine activity; cytokine activity; extracellular matrix binding; fibronectin binding; growth factor activity; growth factor activity; heparin binding; heparin binding; platelet-derived growth factor receptor binding; protein binding; protein heterodimerization activity; protein homodimerization activity; receptor agonist activity; vascular endothelial growth factor receptor 1 binding; vascular endothelial growth factor receptor 2 binding; vascular endothelial growth factor receptor binding