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Recombinant Rat Chemokine (C-C Motif) Ligand 3

Cat. No.: Ccl3-56R
Product Overview: Recombinant Rat Macrophage Inflammatory Protein-1 alpha produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7853 Dalton. The MIP-1 is purified by proprietary chromatographic techniques.
Description: Macrophage Inflammatory Proteins (MIP) belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1α and MIP-1β that are now officially named CCL3 and CCL4 respectively. Both are major factors produced by macrophages after they are stimulated with bacterial endotoxins. They activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. They also induce the synthesis and release of other pro-inflammatory cytokines such as interleukin 1 (IL-1), IL-6 and TNF-α from fibroblasts and macrophages.
Source: Escherichia Coli.
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Pro-Tyr-Gly-Ala.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation: The protein was lyophilized from 1 mg/ml solution containing no additives.
Specific Activity: The Activity is calculated by the ability to chemoattract of rat peritoneal macrophages using a conc. of 60 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1a in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.38 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of MIP-1 as a Reference Standard.
Stability: Lyophilized MIP-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution CCL3 should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name: Ccl3 chemokine (C-C motif) ligand 3 [ Rattus norvegicus ]
Synonyms: Ccl3; chemokine (C-C motif) ligand 3, Scya3; MIP-1a; macrophage inflammatory protein 1 alpha (Small inducible cytokine A3); small inducible cytokine A3
Gene ID: 25542
mRNA Refseq: NM_013025
Protein Refseq: NP_037157
UniProt ID: P50229
Chromosome Location: 10q26
Pathway: Chemokine signaling pathway; Cytokine-cytokine receptor interaction; Toll-like receptor signaling pathway
Function: chemoattractant activity;chemokine activity; heparin binding; protein binding

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