Cat. No. : |
NGB-127H |
Description : |
Neuroglobin, 151 amino acid residue protein, mainly expressed in vertebrate brain and retina, is a recently identified member of the globin superfamily. Augmenting O (2) supply, neuroglobin promotes survival of neurons upon hypoxic injury, potentially limiting brain damage. Moreover, neuroglobin may be a novel oxidative stress-responsive sensor for signal transduction in the brain. Neuroglobin expression is increased by neuronal hypoxia in vitro and focal cerebral ischemia in vivo, and neuronal survival after hypoxia is reduced by inhibiting neuroglobin expression with an antisense oligo deoxynucleotide and enhanced by neuroglobin overexpression. |
Source : |
Escherichia Coli. |
Amino Acid Sequence : |
MERPEPELIR QSWRAVSRSP LEHGTVLFAR LFALEPDLLP LFQYNCRQFS SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLA SLGRKHRAVG VKLSSF STVG ESLLYMLEKC LGPAFTPATR AAWSQLYGAV VQAMSRGWDGE. |
Specificity : |
The amino acid sequence of the Neuroglobin human recombinant is 100% homologous to the amino acid sequence of the human Neuroglobin. |
Purification Method : |
Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding. |
Purity : |
>95% (SDS-PAGE analyzed). |
Formulation : |
Sterile filtered and lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH-4. |
Reconstitution : |
Add 0.2 ml of H2O and let the lyophilized pellet dissolve completely. |
Solubility/Shelf Life : |
The lyophilized protein remains stable until the expiry date when stored at -20°C. |
Applications : |
Western blotting. |
Storage : |
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |