Recombinant human Activin A, Active, His-tagged

• Cat.No.: INHBA-1542H
• Product Overview: Recombinant human Activin A is a polypeptide chain containing 116 amino acids (311–426 P08476 INHBA_HUMAN) and a His-tag at the N-terminal end. Several forms are observed with different predicted molecular masses such as monomer (13.7 kDa), dimmer ( 27.4 kDa) and multimeric forms.

Specification

• Description: Activins are homodimers or heterodimers of the various β subunit isoforms, belonging to the TGFβ family. Mature Activin A has two 116 amino acids residues βA subunits (βA-βA). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells known to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells.As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B. The biological activity of Activin A can be neutralized by inhibins and by the diffusible TGF-B antagonist, Follistatin.
• Source: Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants. This product contains no animal–derived components or impurities. Animal Free product.
• Species: Human
• Tag: His
• Form: Recombinant human Activin is lyophilized from a Tris HCl 0.05M buffer at pH 7.4.
• Molecular Mass: Monomer (13.7 kDa), dimmer ( 27.4 kDa)
• AA Sequence: HHHHHHGLECDGKVNICCKKQFFVS FKDIGWNDWIIAPSGYHANYCEGEC PSHIAGTSGSSLSFHSTVINHYRMR GHSPFANLKSCCVPTKLRPMSMLYY DDGQNIIKKDIQNMIVEECGCS
• Endotoxin: < 0.04 eu/μg protein (lal>
• Purity: >97% by SDS-PAGE gel
• Storage: This lyophilized preparation is stable at 2-8o C for short term, long storage it should be kept at -20oC. Reconstituted protein should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended.
• Reconstitution: Centrifuge vial before opening. Lyophilized protein should be reconstituted in water to a concentration of 50 ng/μl. Due to the protein nature, dimmers and multimers may be observed. At higher concentration the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines.

Gene Information

• Gene Name: INHBA inhibin, beta A [ Homo sapiens ]
• Official Symbol: INHBA
• Synonyms: INHBA; inhibin, beta A; inhibin, beta A (activin A, activin AB alpha polypeptide); inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; FSH-releasing protein; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; EDF; FRP
• Gene ID: 3624
• mRNA Refseq: NM_002192
• Protein Refseq: NP_002183
• MIM: 147290
• UniProt ID: P08476
• Chromosome Location: 7p15-p13
• Pathway: ALK1 signaling events, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Glycoprotein hormones, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Peptide hormone biosynthesis, organism-specific biosystem
• Function: cytokine activity; follistatin binding; growth factor activity; hormone activity; identical protein binding; protein binding; protein heterodimerization activity; type II activin receptor binding